Protein Info for LU632_RS13070 in Erwinia tracheiphila SCR3

Annotation: D-amino acid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF00070: Pyr_redox" amino acids 2 to 36 (35 residues), 24.7 bits, see alignment 1e-08 PF01266: DAO" amino acids 2 to 396 (395 residues), 279.1 bits, see alignment E=2.7e-86 PF02558: ApbA" amino acids 3 to 33 (31 residues), 24.5 bits, see alignment (E = 6.3e-09) PF13450: NAD_binding_8" amino acids 5 to 44 (40 residues), 30.9 bits, see alignment 9.7e-11

Best Hits

Swiss-Prot: 81% identical to DADA_ERWT9: D-amino acid dehydrogenase (dadA) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 85% identity to ebi:EbC_24320)

MetaCyc: 73% identical to D-amino acid dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-11193 [EC: 1.4.5.1]; 1.4.5.- [EC: 1.4.5.1]

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.5.1 or 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (431 amino acids)

>LU632_RS13070 D-amino acid dehydrogenase (Erwinia tracheiphila SCR3)
MRVVILGSGVVGVASAWYLAKAGHDVTVIDRQPGVAMETSAGNAGQISPGYAAPWAAPGV
PLKAIKWMFQRHAPLAIRPDGSLFQLEWMWQMLKNCNMQHYQQNKSRMVRIAEYSRDCIK
ALRDETGIAYEGRQQGTLQLFRTAQQFESASKDIAVLKDAGVPYQLLEASQLADAEPALA
CVQHKLTGGLRFPNDETGDCHLFTLRLAEMAVAEGVNFKFNMPVNALLREDNKIVGVMCG
GATVTADAYVVAAGVFSTSLLQDIVAVPVYPLKGYSLTIPIKNRTIAPLSTVLDETYKVA
ITRFDDRIRVGGMAEIVGYDTALCEARRKTLEMVVGELFPHAGAIEQANFWTGLRPMTPD
GAPIVGRTPLKNLYLNTGHGTLGWTMTCGSGQLISDIISGRNTAIDADDLSVMRYLPGFE
LGVDQSFSLAR