Protein Info for LU632_RS12255 in Erwinia tracheiphila SCR3

Annotation: exoribonuclease II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 643 TIGR02062: exoribonuclease II" amino acids 1 to 641 (641 residues), 1076.2 bits, see alignment E=0 TIGR00358: VacB and RNase II family 3'-5' exoribonucleases" amino acids 6 to 640 (635 residues), 740.7 bits, see alignment E=1.4e-226 PF08206: OB_RNB" amino acids 24 to 80 (57 residues), 60.1 bits, see alignment 2.7e-20 PF00773: RNB" amino acids 189 to 515 (327 residues), 286.4 bits, see alignment E=6.6e-89 PF00575: S1" amino acids 559 to 637 (79 residues), 31.7 bits, see alignment E=3.3e-11

Best Hits

Swiss-Prot: 81% identical to RNB_ERWT9: Exoribonuclease 2 (rnb) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K01147, exoribonuclease II [EC: 3.1.13.1] (inferred from 85% identity to ebi:EbC_23480)

MetaCyc: 76% identical to RNase II (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; 3.1.13.1 [EC: 3.1.13.1]; 3.1.13.1 [EC: 3.1.13.1]

Predicted SEED Role

"Exoribonuclease II (EC 3.1.13.1)" in subsystem RNA processing and degradation, bacterial (EC 3.1.13.1)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (643 amino acids)

>LU632_RS12255 exoribonuclease II (Erwinia tracheiphila SCR3)
MFQDNPLLAQLKEKLHSQTPRVEGVVKGTEKGFGFLEVDPQKSYFIPPPFMKKVMHGDRV
VAVLQTDKDREVADPETLVEPFLSRFVGRIQKKDDRLSIVPDHPLLKDAIQCRADRNLKH
DFQNGDWAVAEMRRHPLKGDRTFYAELTAFVTKGDDHLAPWWVTLSRHNLEREAPEGVAV
EMIDENLTREDLTALEFVTIDSASTEDMDDALYVEETADGELSLTIAIADPTAYVAAGSP
LDDIAAQRAFTNYLPGFNIPMLPRQLSDDVCSLRPHQRRPVMACRVTVADDGSMGEPHFF
AAWIESKAKLVYDNVSDWLENSGEWQPETEFIARQIELLHRLCLSRVAWRQTHALVFKDR
PDFRFLLGEKGEVLDIIAEHRRIANRIVEEAMIAANICAASVLRDQLGFGIYNIHPGFDA
ANAELAAAVLANHGVQADPLAIATLDGFRVLRRELDAQPTQFLDSRIRRFQSFAEIGTEP
GPHFGLGLEAYATWTSPIRKYGDMINHRLLKSIVRGEKAERPQDDITIRMSERRRLNRMA
ERDVGDWLYSRFLQSAAGTDQRFSAEIIDISRGGMRVRLTDNGAVAFVPAPFIHAVRDEL
VCSQENGTAQIKGEVAYRVTDIIDVTVAEVRIETRSIVARPAA