Protein Info for LU632_RS11260 in Erwinia tracheiphila SCR3

Name: tyrS
Annotation: tyrosine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 TIGR00234: tyrosine--tRNA ligase" amino acids 2 to 424 (423 residues), 463.5 bits, see alignment E=3.4e-143 PF00579: tRNA-synt_1b" amino acids 31 to 329 (299 residues), 309.9 bits, see alignment E=9.3e-97

Best Hits

Swiss-Prot: 87% identical to SYY_SALAR: Tyrosine--tRNA ligase (tyrS) from Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 90% identity to ebi:EbC_19330)

MetaCyc: 85% identical to tyrosine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Tyrosine--tRNA ligase. [EC: 6.1.1.1]; 6.1.1.1 [EC: 6.1.1.1]

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (424 amino acids)

>LU632_RS11260 tyrosine--tRNA ligase (Erwinia tracheiphila SCR3)
MASSNLIKQLQERGLVAQVTDENALAERLAQGPVALYCGFDPTADSLHLGHLVPLLCLKR
FQDAGHRPVALVGGATGMIGDPSFKADERKLNTAETVGEWVEKIRRQVAPFLDFECGSNS
AIAANNYDWFGQMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDQGISFTEFSYNLLQG
YDFACLNERHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKSDGTKFGKTE
GGAVWLDAKKTSPYKFYQFWINTADADVYRFLRFFTFMSLDEINALEEEDKKSGKAPRAQ
YVLAQQVTRLVHGAAGLAAAERITNSLFSGSLDEMTEADFQQLAQDGVPFVELTQGQDLQ
QAMVDGELAPSRGQARKLIEASAVSINGVVQPDSEYVFNAAERLHGRYTLLRRGKKNYCL
IVWK