Protein Info for LU632_RS09955 in Erwinia tracheiphila SCR3

Name: pyrD
Annotation: quinone-dependent dihydroorotate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 3 to 336 (334 residues), 516 bits, E=2e-159
• PF01180: DHO_dh
  • amino acids 47 to 335 (289 residues), 289.5 bits, E=1.3e-90

Best Hits

Swiss-Prot: 84% identical to PYRD_ERWT9: Dihydroorotate dehydrogenase (quinone) (pyrD) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 86% identity to ebi:EbC_15300)

MetaCyc: 82% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Protein Sequence (336 amino acids)

>LU632_RS09955 quinone-dependent dihydroorotate dehydrogenase (Erwinia tracheiphila SCR3)
MMYNLVRHALFQLDAERAHELTLQQLHRIAGTPLIHLVRQTLPFKPVNCMGLTFKNALGL
AAGLDKNGECIDAFGAMGFGFIEVGTVTPRPQPGNNKPRMFRLREAEGIINRMGFNNLGV
DNLVGNVKKSHYDGILGINIGKNKDTSVEQGKEDYLICMDKIYPYAGYIAINISSPNTPG
LRTLQYGEALDDLLQALKQKQKDLETRHHKYVPLAVKIAPDLSEEELIQIADSLVRHNID
GVIATNTTLDRSLVTGLKHSEESGGLSGRPVQLRSTEVIRRLATEFNGHLPIIGVGGIDS
LISAREKMAAGASLVQIYSGFIYKGPPLIKEIATHL