Protein Info for LU632_RS09865 in Erwinia tracheiphila SCR3

Name: cmoM
Annotation: tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 262 transmembrane" amino acids 147 to 168 (22 residues), see Phobius details PF13489: Methyltransf_23" amino acids 28 to 160 (133 residues), 50 bits, see alignment E=8.7e-17 PF13847: Methyltransf_31" amino acids 45 to 151 (107 residues), 61.5 bits, see alignment E=2.4e-20 PF01209: Ubie_methyltran" amino acids 45 to 148 (104 residues), 28.7 bits, see alignment E=2.5e-10 PF13649: Methyltransf_25" amino acids 48 to 143 (96 residues), 60.8 bits, see alignment E=5e-20 PF08241: Methyltransf_11" amino acids 49 to 146 (98 residues), 62.6 bits, see alignment E=1.3e-20 PF08242: Methyltransf_12" amino acids 49 to 145 (97 residues), 59.5 bits, see alignment E=1.3e-19

Best Hits

Swiss-Prot: 61% identical to CMOM_ECO57: tRNA 5-carboxymethoxyuridine methyltransferase (cmoM) from Escherichia coli O157:H7

KEGG orthology group: K06219, S-adenosylmethionine-dependent methyltransferase (inferred from 84% identity to ebi:EbC_15130)

MetaCyc: 61% identical to tRNA cmo5U34 methyltransferase (Escherichia coli K-12 substr. MG1655)
2.1.1.M28 [EC: 2.1.1.M28]

Predicted SEED Role

"S-adenosylmethionine-dependent methyltransferase Functionally Coupled to the MukBEF Chromosome Partitioning Mechanism" in subsystem MukBEF Chromosome Condensation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.M28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (262 amino acids)

>LU632_RS09865 tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM (Erwinia tracheiphila SCR3)
MQDRNFDDIAEKFSQNIYGTTKGRIRQAIVRQELDDILASLPTGPLRVLDAGGGEGRTSC
QLAALGHRVLLCDLSEEMLNRARTHAAEKGVSENMQFKQISAQQISQDLDKPADLVLFHA
VLEWVADPEAVLVALYDALVPGGVLSLMFYNVNGLMMQTLVLGNFGYMQADLRKRKRKTL
SPDHPRDPEQVYGWLEDVGFVVENKTGIRVFHDYMRDRHKQTERFEEILALEKRFCRQEP
FLSLGRYIHVTARKPINRNNHE