Protein Info for LU632_RS09760 in Erwinia tracheiphila SCR3

Name: serS
Annotation: serine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02403: Seryl_tRNA_N
  • amino acids 1 to 107 (107 residues), 111.7 bits, E=2.1e-36
• TIGR00414: serine--tRNA ligase
  • amino acids 1 to 423 (423 residues), 584.2 bits, E=8e-180
• PF00587: tRNA-synt_2b
  • amino acids 229 to 406 (178 residues), 149 bits, E=1.5e-47

Best Hits

Swiss-Prot: 86% identical to SYS_ERWT9: Serine--tRNA ligase (serS) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K01875, seryl-tRNA synthetase [EC: 6.1.1.11] (inferred from 91% identity to ebi:EbC_14940)

MetaCyc: 82% identical to serine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Serine--tRNA ligase. [EC: 6.1.1.11]; 6.1.1.11 [EC: 6.1.1.11]

Predicted SEED Role

"Seryl-tRNA synthetase (EC 6.1.1.11)" in subsystem Glycine and Serine Utilization (EC 6.1.1.11)

MetaCyc Pathways

• superpathway of chorismate metabolism (50/59 steps found)
• tRNA charging (21/21 steps found)
• L-selenocysteine biosynthesis I (bacteria) (2/3 steps found)
• L-selenocysteine biosynthesis II (archaea and eukaryotes) (1/4 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• enterobactin biosynthesis (4/11 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.11

Protein Sequence (430 amino acids)

>LU632_RS09760 serine--tRNA ligase (Erwinia tracheiphila SCR3)
MLDPNLLRNEPDAVAEKLARRGFKLDVETLRLQEERRKVLQVETETLQAERNARSKTIGQ
AKVRGEDIEPLLQEVNQLGERLEAAKAELDTLQSEIRDLALTIPNIPADEVPPGKDDSEN
LEISRWGEPRHFDFPVRDHVELGEMAHGLDFAMGAKLTGSRFVVMQGQIALMHRALSQFM
LDLHTEQHGYLETYVPYLVNHASLYGTGQLPKFGEDLFHTRPLEEEASSSDYALIPTSEV
PLTNMVRDEILEEESLPLKFTAHTPCFRSEAGAYGRDTRGLIRMHQFDKVEMVQIARPEE
SMQALEEMVGHAEKVLQLLQLPYRKVLLCTGDMGFGSGKTYDLEVWLPAQDTYREISSCS
NMGDFQARRMQARCRSKTEKKPRLVHTLNGSGLAVGRALVAVLENYQQADGRIAVPEVLK
KYMRGLEYIG