Protein Info for LU632_RS08945 in Erwinia tracheiphila SCR3

Name: hisD
Annotation: histidinol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 PF00815: Histidinol_dh" amino acids 30 to 428 (399 residues), 539.4 bits, see alignment E=3.1e-166 TIGR00069: histidinol dehydrogenase" amino acids 37 to 427 (391 residues), 525.8 bits, see alignment E=4.1e-162

Best Hits

Swiss-Prot: 81% identical to HISX_SALTY: Histidinol dehydrogenase (hisD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00013, histidinol dehydrogenase [EC: 1.1.1.23] (inferred from 85% identity to ebi:EbC_29100)

MetaCyc: 81% identical to histidinal/histidinol dehydrogenase (Escherichia coli K-12 substr. MG1655)
Histidinol dehydrogenase. [EC: 1.1.1.23]

Predicted SEED Role

"Histidinol dehydrogenase (EC 1.1.1.23)" in subsystem Histidine Biosynthesis (EC 1.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (435 amino acids)

>LU632_RS08945 histidinol dehydrogenase (Erwinia tracheiphila SCR3)
MTDFATPIDWQTCTPEQQQALLSRPALSASDSIALTVRDIIEKVKAEGDAALRHYSATFD
KTDVTALRVTEQQIADATARLSDEIKQAMVVAVGNIETFHLAQRLPPVDIETQPGVRCQQ
ITRPLASVGLYIPGGSAPLFSTVLMLATPARIAGCQRVVLCSPPPIADEILYAATLCGVE
EIFQAGGAQGIAALALGTESVPKVDKIFGPGNAYVTEAKRQVSQRLDGAAIDMPAGPSEV
LVIADDEATPDFVASDLLSQAEHGPDSQVILLTPSLQMAEAVAAAVAQQLTTLPRAETAR
KALESSRLIVAEDMPQCVEISNRYGPEHLIIQTRNARDWVGHITSAGSIFLGDWSPESAG
DYASGTNHVLPTYGYTSTCSSLGLADFQKRMTVQELTKEGFRALAPTIEILAAAEQLIAH
KNAVTLRLNALKEQK