Protein Info for LU632_RS08590 in Erwinia tracheiphila SCR3

Name: thiD
Annotation: bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00097: hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
  • amino acids 6 to 262 (257 residues), 341.1 bits, E=1.7e-106
• PF08543: Phos_pyr_kin
  • amino acids 13 to 260 (248 residues), 312.5 bits, E=1e-97

Best Hits

Swiss-Prot: 77% identical to THID_SALTY: Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (thiD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00941, hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [EC: 2.7.1.49 2.7.4.7] (inferred from 78% identity to ebi:EbC_29680)

MetaCyc: 77% identical to bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (Escherichia coli K-12 substr. MG1655)
Phosphomethylpyrimidine kinase. [EC: 2.7.4.7]; Hydroxymethylpyrimidine kinase. [EC: 2.7.4.7, 2.7.1.49]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7)" (EC 2.7.4.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• hydroxymethylpyrimidine salvage (2/2 steps found)
• thiamine diphosphate salvage II (4/5 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
• thiamine diphosphate salvage IV (yeast) (3/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (3/8 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (2/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.49 or 2.7.4.7

Protein Sequence (266 amino acids)

>LU632_RS08590 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (Erwinia tracheiphila SCR3)
MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGTSVITALVAQNTCGVQAFHRVEPDFV
AAQLASVLSDVRIDSAKIGMLSDSDIVEVVADRLRTAAIPWVVLDTVMLAKSGDALLSPQ
AMTTLRQRLLPQVSLITPNLPEAAALLACDIARSESEMRQQGRALRTMGCRAVLMKGGHL
DEAESPDWLFTAEDEQRFSSPRINTQNTHGTGCTLSAALAALRPRHSGWHDTVVQAKRWL
QGALEQADSLEVGKGQGPVHHFYRWW