Protein Info for LU632_RS08570 in Erwinia tracheiphila SCR3

Name: metG
Annotation: methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 677 PF00133: tRNA-synt_1" amino acids 8 to 84 (77 residues), 42.3 bits, see alignment E=1.3e-14 TIGR00398: methionine--tRNA ligase" amino acids 9 to 535 (527 residues), 662.7 bits, see alignment E=4.2e-203 PF09334: tRNA-synt_1g" amino acids 9 to 396 (388 residues), 542.9 bits, see alignment E=1.2e-166 PF19303: Anticodon_3" amino acids 415 to 539 (125 residues), 34.3 bits, see alignment E=6.5e-12 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 549 to 676 (128 residues), 143 bits, see alignment E=5.3e-46 PF01588: tRNA_bind" amino acids 581 to 674 (94 residues), 78.4 bits, see alignment E=1.1e-25

Best Hits

Swiss-Prot: 88% identical to SYM_ERWT9: Methionine--tRNA ligase (metG) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 87% identity to eam:EAMY_2279)

MetaCyc: 84% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (677 amino acids)

>LU632_RS08570 methionine--tRNA ligase (Erwinia tracheiphila SCR3)
MAQVAKKLLVTCALPYANGPIHLGHMLEHIQADIWVRYQRMRGNEVYFICADDAHGTPIM
LKAQQQGVTPEQMIAEMSQEHQTDFAGFNISYDNYHSTHSDENRELSTLIYSCLKENGFI
KNRTISQLYDPEKDMFLPDRFVKGTCPKCKAVDQYGDNCEVCSATYSPAELINPKSAVSG
ATPVMRDSEHFFFDLPEFGTMLQAWTRSGALQAQVANKMQEWFAAGLQQWDISRDAPYFG
FEIPDAPGKYFYVWLDAPVGYMGSFKNLCDRRGDIDFDAFWRKDSNAGLYHFIGKDIVYF
HSLFWPAMLEGSRFRKPTNLFVHGYVTVNGAKMSKSRGTFIKASTWLQHLDADSLRYYYA
ARLSPHIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFITKRFDGKLSAELADPTLYKT
FTDASARIAEAWESREFSRAIRDIMALADAANRYVDEQAPWTVAKQKGREADLQAICSMG
INLFRVLMTWLKPVLPGLSERAEAFLNQSLNWDDIEQPLLGHTVAPFKALYQRIEMDKVN
ALLDASKQDNAAANKQALSRPLANAPLQETITFDDFAKVDMRIALIQNAEFVDGSDKLLR
LTLDLGGETRNVFSGIRAAYPDPAVLNGRLTVMVANLAPRKMRFGISEGMVMAAGPGGSD
IFLLSPDSGAQPGMQVT