Protein Info for LU632_RS08025 in Erwinia tracheiphila SCR3

Name: nuoN
Annotation: NADH-quinone oxidoreductase subunit NuoN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01770: proton-translocating NADH-quinone oxidoreductase, chain N
  • amino acids 9 to 475 (467 residues), 463.8 bits, E=3.8e-143
• PF00361: Proton_antipo_M
  • amino acids 122 to 420 (299 residues), 288.1 bits, E=3.6e-90

Best Hits

Swiss-Prot: 89% identical to NUON_ERWT9: NADH-quinone oxidoreductase subunit N (nuoN) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K00343, NADH dehydrogenase I subunit N [EC: 1.6.5.3] (inferred from 93% identity to ebi:EbC_30640)

MetaCyc: 83% identical to NADH:quinone oxidoreductase subunit N (Escherichia coli K-12 substr. MG1655)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]; 7.1.1.- [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NADH to fumarate electron transfer (2/2 steps found)
• aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NADH to dimethyl sulfoxide electron transfer (1/2 steps found)
• NADH to hydrogen peroxide electron transfer (1/2 steps found)
• NADH to trimethylamine N-oxide electron transfer (1/2 steps found)
• nitrate reduction VIII (dissimilatory) (1/2 steps found)
• aerobic respiration I (cytochrome c) (2/4 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• Fe(II) oxidation (1/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Protein Sequence (485 amino acids)

>LU632_RS08025 NADH-quinone oxidoreductase subunit NuoN (Erwinia tracheiphila SCR3)
MTITPQQLIALLPMLIVGLTVVVVMLSIAWRRNHFVNATLAVVGLNLALLSLFFVAKVGP
MDVTPLLRVDGYSMLYTGLVILASLATCTFAYPWLATYPDNREEFYLLALIAALGGVVLA
SANHLASMFIGIELISLPLFGLVGYAFQQKRSLEAGIKYTIMSAVASSFLLFGIALLYAD
SGNLSFVALGKSLNDTMVHQPLLLAGLGMMIVGLGFKLSLVPFHLWTPDVYQGAPAPVST
FLATASKIAIFGVVMRLFLYAPVANSESVRLVLAIIAFASILFGNLMAISQSNIKRLLGY
SSIAHLGYLLVALIAIQTHQLSLEAVGIYLAGYLFSSLGAFGVVSLMSSPYRGPDADSLY
SYRGLFWHRPILSAVMTVMLLSLAGIPMTLGFIGKFYVIAVGVGAHLWWLTGAVVVGSAI
GLYYYLRVTVSLYLTPPELLKRDTPANWAFTAGGVVVLISAVLVLLLGIYPQPLISLVQM
AQPLM