Protein Info for LU632_RS07850 in Erwinia tracheiphila SCR3

Name: folC
Annotation: bifunctional tetrahydrofolate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 30 to 412 (383 residues), 325.1 bits, E=4.4e-101
• PF08245: Mur_ligase_M
  • amino acids 52 to 194 (143 residues), 37.4 bits, E=2.7e-13
• PF02875: Mur_ligase_C
  • amino acids 284 to 335 (52 residues), 25.4 bits, 1.4e-09

Best Hits

Swiss-Prot: 66% identical to FOLC_ECOLI: Dihydrofolate synthase/folylpolyglutamate synthase (folC) from Escherichia coli (strain K12)

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 73% identity to ebi:EbC_31080)

MetaCyc: 66% identical to bifunctional folylpolyglutamate synthetase / dihydrofolate synthetase (Escherichia coli K-12 substr. MG1655)
Tetrahydrofolate synthase. [EC: 6.3.2.17]; 6.3.2.17 [EC: 6.3.2.17]; 6.3.2.17 [EC: 6.3.2.17]; Dihydrofolate synthase. [EC: 6.3.2.17, 6.3.2.12]

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) / Folylpolyglutamate synthase (EC 6.3.2.17)" in subsystem Folate Biosynthesis (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of chorismate metabolism (50/59 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.12, 6.3.2.17

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (420 amino acids)

>LU632_RS07850 bifunctional tetrahydrofolate synthase/dihydrofolate synthase (Erwinia tracheiphila SCR3)
MDTIPQATSSLVTWLHYLENLHGQSVDPGLERIAQVAQQLDLLTPAPFIFTVAGTNGKGT
SCRMLETLLLAAGYRVGVYSSPHLLRYTERVRVQGAELAESDHTASFARIEAGRGNISLT
YFEYSTLSALALFKQLALDVVILEVGLGGRLDATNIVNADIAVITSIALDHTDYLGPDRE
SIGREKAGIFRSGKPAVVGEPDMPLSIAEVAKSVGATLLREGQAWSYTVGENDWCFHDDR
GDLAGLPLPQIPLPNAATVLAALRASSLDIDPAIIRHWLDKVVVTGRFQTISDSPRIILD
VAHNPHAAAWLAGQLGRLAKNGSVHAVVGMLHDKDIPGTLACLKPQIDRWYCAPLDGPRG
ARADELMAHLTDADAFSCVADAFAHARRQALAQDIVLVCGSFHTVGQVMEMLEMEKGSGK