Protein Info for LU632_RS06400 in Erwinia tracheiphila SCR3
Name: cysJ
Annotation: NADPH-dependent assimilatory sulfite reductase flavoprotein subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 76% identical to CYSJ_SHIBS: Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ) from Shigella boydii serotype 4 (strain Sb227)
KEGG orthology group: K00380, sulfite reductase (NADPH) flavoprotein alpha-component [EC: 1.8.1.2] (inferred from 84% identity to ebi:EbC_35390)MetaCyc: 76% identical to sulfite reductase, flavoprotein subunit (Escherichia coli K-12 substr. MG1655)
Sulfite reductase (NADPH). [EC: 1.8.1.2]; Flavin reductase. [EC: 1.8.1.2, 1.5.1.30]
Predicted SEED Role
"Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)
MetaCyc Pathways
- superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
- assimilatory sulfate reduction I (4/4 steps found)
- assimilatory sulfate reduction III (3/3 steps found)
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (9/12 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (6/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.5.1.30, 1.8.1.2
Use Curated BLAST to search for 1.5.1.30 or 1.8.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (597 amino acids)
>LU632_RS06400 NADPH-dependent assimilatory sulfite reductase flavoprotein subunit (Erwinia tracheiphila SCR3) MTTQVPPGSLLPLSSEQLARLQAATGDFSTTQLAWLSGYFWGMVNQVPASAPAPSEIPGI TLLSASQTGNARRLAEQLRDDLLAVKLNVNLVNSGDFKFKQIAQEKLLVVVTSTQGEGEP PEEAVALHKFLMSKKAPKLDNTAFAVFGLGDSSYAFFCQAGKDFDNRLAELGGERLLARA DADVEYAAEAERWRSQIVEILKSRVPTAASVQAAANAAGSVNHVSSSPWNKETPLTASLS VNQKITGRNSDKDVRHIEIDLGDSGLRYQPGDALGVWYENDPALVKELLALLELKGDESV ALNGETLPLAEALQKHLELTVNTPQIVEQYGQLSQHEPLLSLASDKSQLQHYAQSVPIVD MVRRAPIVLKAEQLLAMLRPLTPRLYSIASSQAENETEVHITVGAVRYEIDGRPRAGGAS SFLADRLEEDGELRIFIEHNDNFRLPADPRAPVIMIGPGTGIAPFRAFLQQRDNDGAEGK NWLFFGNPHFTEDFLYQVEWQRYVKDGLLTHIDLAWSRDQQHKVYVQDKIRARGAEVWRW IEQGAHIYVCGDANRMAKDVEQALLEVVAEHGGMDLDTADEFLSELRIERRYQRDVY