Protein Info for LU632_RS06350 in Erwinia tracheiphila SCR3

Name: relA
Annotation: GTP diphosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 742 PF13328: HD_4" amino acids 46 to 191 (146 residues), 143.3 bits, see alignment E=1.7e-45 TIGR00691: RelA/SpoT family protein" amino acids 64 to 740 (677 residues), 624.7 bits, see alignment E=1e-191 PF04607: RelA_SpoT" amino acids 251 to 361 (111 residues), 145.4 bits, see alignment E=2.4e-46 PF02824: TGS" amino acids 408 to 467 (60 residues), 76.5 bits, see alignment 4e-25 PF19296: RelA_AH_RIS" amino acids 486 to 643 (158 residues), 45.1 bits, see alignment E=3.9e-15 PF13291: ACT_4" amino acids 664 to 740 (77 residues), 62.9 bits, see alignment E=1.1e-20 PF01842: ACT" amino acids 668 to 733 (66 residues), 42.1 bits, see alignment E=1.8e-14

Best Hits

Swiss-Prot: 85% identical to RELA_ECO57: GTP pyrophosphokinase (relA) from Escherichia coli O157:H7

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 92% identity to eam:EAMY_0735)

MetaCyc: 85% identical to GDP/GTP pyrophosphokinase (Escherichia coli K-12 substr. MG1655)
GTP diphosphokinase. [EC: 2.7.6.5]; 2.7.6.5 [EC: 2.7.6.5]

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (742 amino acids)

>LU632_RS06350 GTP diphosphokinase (Erwinia tracheiphila SCR3)
MVAVRSAHLNTAGEFALDQWITSLGISNQQSCERLAETWRYCQEATQHHIDAALLLWRGV
EMVEILSMLSMDSESLRAALLFPLADSGVVPEAVLEATFGKAIVSLVHGVRDMDAIRQLK
ATHNDSMASEQVDNVRRMLLAMVEDFRCVVIKLAERIAHLREVKEAPEDERVLAAKECTN
IYAPLANRLGIGQLKWELEDFCFRYLHPDEYKRIAKLLHERRIDREQYIDTFVQSLRDEI
VKQGVKAEVYGRPKHIYSIWRKMQKKSLSFDELFDVRAVRIVAERLQDCYGALGIVHTLY
RHLPSEFDDYVANPKPNGYQSIHTVVLGPKGKTVEIQIRTRQMHENAELGVAAHWKYKEG
GASGSSRGASGHEERIAWLRKLIAWQEEMSDSGEMLDEVRSQVFDDRVYVFTPKGDVVDL
PAGSTPLDFAYHIHSDIGHRCIGAKIGGRIVPFTYQLQMGDQIDIITQKQPNPSRDWLNP
NLGYITTSRGRSKIHAWFRKQDRDKNIVAGRQILDSELDHLDISLKEAEKLLLPRYNFNS
LDELLAAIGGGDIRLNQMTNFLQSKLNKSSAEEEDREALRQLTQKSSAPRSKESGRVVVE
GVGNLMHHIARCCQPIPGDEIVGFITQGRGISIHRADCDQFSELMSHAPERIVDAVWGES
YSSGYLLVVRVTANDRSGLLRDITTILANEKVNVLGVSSRSDTKRQLATIDMDIEIYNQQ
VLGRVLARLNQVPDIIDAKRLH