Protein Info for LU632_RS06275 in Erwinia tracheiphila SCR3

Name: ppnN
Annotation: nucleotide 5'-monophosphate nucleosidase PpnN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14793: DUF4478
  • amino acids 4 to 110 (107 residues), 157.6 bits, E=1.9e-50
• PF18306: LDcluster4
  • amino acids 154 to 264 (111 residues), 30.1 bits, E=6.9e-11
• PF03641: Lysine_decarbox
  • amino acids 188 to 327 (140 residues), 99.2 bits, E=4.3e-32
• PF11892: PpnN_C
  • amino acids 331 to 451 (121 residues), 184.6 bits, E=1.3e-58

Best Hits

Swiss-Prot: 84% identical to PPNN_ECO57: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase (ppnN) from Escherichia coli O157:H7

KEGG orthology group: K06966, (no description) (inferred from 91% identity to eta:ETA_27320)

MetaCyc: 84% identical to nucleotide 5'-monophosphate nucleosidase (Escherichia coli K-12 substr. MG1655)
AMP nucleosidase. [EC: 3.2.2.4]; Inosinate nucleosidase. [EC: 3.2.2.4, 3.2.2.12]; Pyrimidine-5'-nucleotide nucleosidase. [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]; 3.2.2.- [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]

Predicted SEED Role

"Decarboxylase family protein"

MetaCyc Pathways

• adenosine nucleotides degradation III (1/1 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.4

Use Curated BLAST to search for 3.2.2.10 or 3.2.2.12 or 3.2.2.4

Protein Sequence (452 amino acids)

>LU632_RS06275 nucleotide 5'-monophosphate nucleosidase PpnN (Erwinia tracheiphila SCR3)
MITQISPLGSMDLLSQLEVDMLKRTARSDLYQLFRNCSLAVLNSGSQTDSSHELLSRFEN
FDINILRRERGVKLELHNPPEEAFVDGRIIRSLQANLFAVLRDILFVNGQMDSAVQHQKN
DDSVQITNLVFSILRNARALHVGEEPNTVVCWGGHSINEIEYQYCRKVGSQLGLRELNIC
TGCGSGVMEAPMKGAAVGHAQQRYKEGRFIGLTEPSIIAAEPPNPLVNELIIMPDIEKRL
EAFVRIAHGIIIFPGGVGTAEELLYLLGIMINPHNHDQVLPLILTGPKESADYFRVMDKF
IVNTLGEAARQHYTIIIDDAPEVARQMKKAMPKVKEHRRETGDAYSFNWAIRIAPDLQVP
FIPTHENMANLNLSPDQPAEQLAASLRRAFSGIVAGNVKDVDIRAIKEKGPYRLHGAPEI
MRRMDDLLQGFVAQHRMKLPGTAYIPCYEIIK