Protein Info for LU632_RS01945 in Erwinia tracheiphila SCR3

Name: rsgA
Annotation: small ribosomal subunit biogenesis GTPase RsgA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00157: ribosome small subunit-dependent GTPase A
  • amino acids 73 to 335 (263 residues), 303.8 bits, E=4.8e-95
• PF03193: RsgA_GTPase
  • amino acids 98 to 277 (180 residues), 197.6 bits, E=2.2e-62

Best Hits

Swiss-Prot: 82% identical to RSGA_ERWT9: Small ribosomal subunit biogenesis GTPase RsgA (rsgA) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K06949, ribosome biogenesis GTPase [EC: 3.6.1.-] (inferred from 83% identity to eay:EAM_0429)

MetaCyc: 76% identical to ribosome small subunit-dependent GTPase A (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Ribosome small subunit-stimulated GTPase EngC" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.1.15

Use Curated BLAST to search for 3.6.1.- or 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (353 amino acids)

>LU632_RS01945 small ribosomal subunit biogenesis GTPase RsgA (Erwinia tracheiphila SCR3)
MSKNKLSKGQQRRVSANHDRRLQQRSQKPEPDDSLFGDTQDGIVISRFGMHADVEDLAGT
IHRCNIRRTIRSLVTGDRVCWRAGLQEGASVKGIVEAVHERRSVLTRPDMYDGVKPIAAN
IDQIVIVSAILPELSLNIIDRYLVASETLGVEPLLVLNKIDLLDDEDRQFVDEQMDIYRK
IGYQVLMISNRQKESLQTLERSLTGHISIFAGQSGVGKSSLLNNLLGLEHGGEAILTNEV
SDVSGLGQHTTTASRLYHFPHGGDVIDSPGVREFGLWHLEPEQITRGFIEFRQFLGYCKF
RDCKHDTDPGCAIREAMDRDDIDESRFDNYHRILESMSEMQLKTRRSFSERSD