Protein Info for LU632_RS00185 in Erwinia tracheiphila SCR3

Name: glyS
Annotation: glycine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 689 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 689 (689 residues), 1022 bits, see alignment E=0 PF02092: tRNA_synt_2f" amino acids 6 to 551 (546 residues), 709.3 bits, see alignment E=4.2e-217 PF05746: DALR_1" amino acids 581 to 679 (99 residues), 41.7 bits, see alignment E=1.1e-14

Best Hits

Swiss-Prot: 88% identical to SYGB_ERWT9: Glycine--tRNA ligase beta subunit (glyS) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 91% identity to ebi:EbC_45080)

MetaCyc: 84% identical to glycine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (689 amino acids)

>LU632_RS00185 glycine--tRNA ligase subunit beta (Erwinia tracheiphila SCR3)
MTEKTFLVEIGTEELPPKALRNLAESFAANLTAGLDAAGLNHGEVSWYASPRRLALKVAS
LSTAQPDREVDKRGPAIAAAFDANGIPTKAAEGWARGCGITVDQAERLSTDKGEWLLYRA
QVKGASASALLPAMIDTALAKLPIPKLMRWGDSDVQFVRPVHTVTLLLGDESIPATLLGV
QSGRTLRGHRFMGEPEFTIDHADQYPQILLERGKVQADFGARKSQIKAQAEVAAREIGGI
ADLSDSLLEEVASLVEWPVVLTATFEERFLAVPAEALVYTMKGDQKYFPVYDAAGKLLPN
FIFVSNIESTDPQQIIAGNEKVVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQKELGT
LRDKIDRIQALAGWIAGQTGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
GEDEDVAVALNEQYQPRFAGDDLPSSPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGILRIIVEKNLQLDLQTLTEEAVRLYGSKLSNAQVVDEVIDFMLGRFRTWYQEEG
HSVDTIQAVLARRPTRPADFDARMKAVSHFRTLEAAATLAAANKRVSNILAKSSEPLNES
VQASLLKENEEIQLATYVSALSSKLAPWFAEGRYQDALIELAELRSAVDNFFEKVMVNAE
DRDIRINRLTLLAKLRELFLQVADISLLQ