Protein Info for LRK53_RS18685 in Rhodanobacter sp000427505 FW510-R12

Annotation: error-prone DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1021 TIGR00594: DNA polymerase III, alpha subunit" amino acids 3 to 969 (967 residues), 595.4 bits, see alignment E=1.2e-182 PF02811: PHP" amino acids 4 to 131 (128 residues), 59 bits, see alignment E=1.8e-19 PF07733: DNA_pol3_alpha" amino acids 273 to 527 (255 residues), 299.1 bits, see alignment E=8.2e-93 PF17657: DNA_pol3_finger" amino acids 530 to 694 (165 residues), 192.2 bits, see alignment E=1.1e-60 PF14579: HHH_6" amino acids 769 to 856 (88 residues), 71 bits, see alignment E=2e-23 PF01336: tRNA_anti-codon" amino acids 948 to 1003 (56 residues), 27.2 bits, see alignment 7.6e-10

Best Hits

Swiss-Prot: 60% identical to DNAE2_PSEAE: Error-prone DNA polymerase (dnaE2) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 60% identity to pag:PLES_46601)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1021 amino acids)

>LRK53_RS18685 error-prone DNA polymerase (Rhodanobacter sp000427505 FW510-R12)
MYAELHCLSNLSFLRGVASARALFERAAALGYAALAITDECSLAGIVRGLEASRATGVPL
IVGTELVLEDGARLVLLAEDRAGYATLSRLITVARARAAKGAYQARWKDLEGDHQGLLAI
LCTIDRHGTVPADAEGRAKRLATVLPQRAWIGVELHRGDRDAEAIIACAAVGAAAGLPLV
AAGDVHMATRGQRALQDTVTAIRLGMPVAAAAGELFPNGERHLRTPAELQELYAPELLAE
TLRIAAHCHLDLGTLGYRHPVDVVPLAQAPATWLRHLVEEGAATRWPAGTPPALGAQIER
ELALITELGYEAFFLTVHDIVRYARSRGILCQGRGSAANSVVCYALGITEVDPERGHLLF
ERFLSRERAEPPDIDVDFEHERREEVIQYIYGRYGRDRAALAAAITTYRPRSAVRDVGRA
LDLDPVLVDRVAQLLGHWGGMEDVTARLAESGIDTDTPSMQRWLTLAAQLVGTPRHLSQH
VGGFVISAAPLTELVPVEPATMPGRTVIQWDKSDLETMGLLKVDILALGMLSVLRRAFEH
VERHHGTTLSIATIPAEDPATYAMLRRADTVGVFQVESRAQMAMLPRLRPTCFYDLVVQT
AIIRPGPIQGGMVHPYLRRRQGREVASYPSDEVRGVLERTLGVPIFQEQVMQLAVVAAGF
TAGEADQLRRSMGAWERHGTLEHFRARLLSGMTGRGYAPAFADQVFEMIRGFGAYGFPES
HAASFALLAYASAYLKAHYPSAFLAALLNSQPMGFYTTDQLAQDARRHGIEIRPPDVRVS
GWESHLEGEGARAPIRLGLREISGLRAESAARLLQARREAPFKDLSDVARRAALDQRDLP
LLADAGALQSLAGHRHVARWVASGVEATLPLFGGTAEATVALRAPTAGEEMAADYASMGL
STGSHPMALLRSSLLGSSYATLAEAARAGHRKRIRVAGLIGMRQSPPAAGGVTFLTLEDE
TGWLNVVVWRDVAEQCRQALRSAGPVVVDGRIEHADGVTHLIAARVWTAAFAAAALGDTV
A