Protein Info for LRK53_RS16375 in Rhodanobacter sp000427505 FW510-R12

Annotation: 3-isopropylmalate dehydratase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00170: 3-isopropylmalate dehydratase, large subunit
  • amino acids 3 to 470 (468 residues), 658.3 bits, E=2.9e-202
• PF00330: Aconitase
  • amino acids 8 to 462 (455 residues), 534.5 bits, E=1.3e-164

Best Hits

Swiss-Prot: 62% identical to LEUC_BORPA: 3-isopropylmalate dehydratase large subunit (leuC) from Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)

KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 62% identity to bpa:BPP1942)

MetaCyc: 54% identical to isopropylmalate isomerase large subunit (Leptospira interrogans serovar Lai str. 56601)
(R)-2-methylmalate dehydratase. [EC: 4.2.1.35]; 3-isopropylmalate dehydratase. [EC: 4.2.1.35, 4.2.1.33]; 4.2.1.33 [EC: 4.2.1.35, 4.2.1.33]; RXN-7744 [EC: 4.2.1.35, 4.2.1.33]

Predicted SEED Role

"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35

Protein Sequence (470 amino acids)

>LRK53_RS16375 3-isopropylmalate dehydratase large subunit (Rhodanobacter sp000427505 FW510-R12)
MTPMTLYDKLWNSHHVADLADGSSLVYIDLQLLHEVTSPQGFSNLRRAGRAPWRRSSAIA
MPDHNVPTEGRVQGLAGFTDPLARLQVETLEKNCAEFTVPMFRLNDPRQGIVHIVGPEQG
LTLPGMTVACGDSHTTTHGALATLGYGVGSSEVEHVLATQTLRCRKLKNMAVEVNGQFAP
GAGVKDLALHVIGLVGTGGGSGHAMEFRGTAVEAASMEARMTLCNMSVEAGARVGLIGYD
AVTEAYVRGRSYAPVGEMWGRARAYWAGLHSDPGVQYDRRVVIAAGDVPPCVTWGTSPEM
VVPIDGRVPLLESARDDTQRDGWRRALTYMGLEEGQPIAGTPVDKVFIGSCTNSRIEDLR
LAADLARGHHVHARVKRALVVPGSGMVKLQAEREGLDKVFLASGFEWREPGCSMCLGMNP
DRLKPGERCASTSNRNFEGRQGRGGRTHLMSPAMAVAAAIAGALADVREL