Protein Info for LRK53_RS15870 in Rhodanobacter sp000427505 FW510-R12

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 711 PF13328: HD_4" amino acids 41 to 171 (131 residues), 84.7 bits, see alignment E=1.5e-27 TIGR00691: RelA/SpoT family protein" amino acids 50 to 707 (658 residues), 541.9 bits, see alignment E=1.1e-166 PF04607: RelA_SpoT" amino acids 230 to 340 (111 residues), 144.8 bits, see alignment E=3.3e-46 PF02824: TGS" amino acids 381 to 439 (59 residues), 67.5 bits, see alignment 2.1e-22 PF13291: ACT_4" amino acids 630 to 707 (78 residues), 57.1 bits, see alignment E=6.2e-19

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 58% identity to xcv:XCV3244)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (711 amino acids)

>LRK53_RS15870 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Rhodanobacter sp000427505 FW510-R12)
MAPIVEPSSLSRWREHAGAVLPALAEPLEACTTRAVNQVECADVLELLGMLGCDAQTQAA
ALWFELARVDPALWAQRRAALPAELQRLVDGQQAAEQVWALHAQRPPQGAAEGLRRLLLA
IVRDLRVVFVLLARQLARMRAAPALPEDERQALARLTRDIHAPLANRLGIWQLKWELEDL
AFRYLQPGTYRRIANLLDERRADREAFIHDSLDQLQRALAAAGIQAELAGRPKHIYSIWK
KMQRKSLDFSDLYDIRAVRVLVDSITDCYAALGVVHALWPHLPGEFDDYIARPKANGYQS
LHTAVIGPEGKTLEVQIRTHAMHRANELGVAAHWRYKEGGSADAEFEAKIAWMRKLLEPR
GEDDSALAAELQTELLEDRVYLLTPKGEVFDLARGATVLDFAYLVHTEVGHRCRGAKVNG
RIVPLTFQPQSGDRVEILTGKLAEPSRDWLSPHHGYLNTARAKDKVRAWFRKIAHDANLA
VGRGMFERELKRLALASADVTKLPAHFHLKNHDELLVALALGEVTSGQIARVLQEAAQPS
EPAAVTQSGTPVASRHATLDHSALRIEGIGNLLTTLARCCQPLPGDPVRGFVTKGRGVSV
HRTDCSSLARLAARDPDRVIEVSWGNAAAQAYEVDIELRGYDRKGLQKDVTSVVSNAGTH
IIASSSRLFVHTGEVEMRFTLRVRDFEQLSTLLGKLLALPNVLDVRRVGTG