Protein Info for LRK53_RS15845 in Rhodanobacter sp000427505 FW510-R12

Annotation: penicillin-binding protein 1B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 782 transmembrane" amino acids 20 to 39 (20 residues), see Phobius details TIGR02071: penicillin-binding protein 1B" amino acids 29 to 741 (713 residues), 821.4 bits, see alignment E=3.2e-251 PF14814: UB2H" amino acids 66 to 150 (85 residues), 52.7 bits, see alignment E=5.6e-18 PF00912: Transgly" amino acids 156 to 332 (177 residues), 194.8 bits, see alignment E=1.3e-61 PF00905: Transpeptidase" amino acids 425 to 663 (239 residues), 77.5 bits, see alignment E=1.6e-25

Best Hits

KEGG orthology group: K05365, penicillin-binding protein 1B [EC: 2.4.1.129 3.4.-.-] (inferred from 50% identity to xac:XAC3109)

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (782 amino acids)

>LRK53_RS15845 penicillin-binding protein 1B (Rhodanobacter sp000427505 FW510-R12)
MALLTRTRALARRSWPWLRIPFWIGMGLLFGFVLPYTLVLNKRVQDRFNELVFAVPTRVY
ARPLPLAADTPMTSAALELELTFAGYSNDGKGQVAGSWVKQGARYTIASRGYAGPDGGEL
PKRIRVTLGQGVVQSVQDAASGKPIELAHLDPARIATLYGASQEERRFVRLADVPPLLVS
GLQAVEDRDFNHHIGIDVSAIARAAFANLRAGHTVQGGSTLTQQLVRNLFLSREQNFTRK
INEALMALLLEAHYSKGRILEAYVNDVFLGQQGGQAVHGFAAASEFYFGRRLEDLRPQEI
ALLVGMVKGPSYYDPRRSPQRALARRNLVLQEFFDTNLIGAGQLNTAQAAPLDIVKNGQL
PHNRFPAFMDLVRKQITADFDEQALREGKLSIFTTLDPAAQLYAERAITDSLKGLGKRGD
GVQAAAVVTSAQTGSVLAVVGSRTPGEQGFNRALDARRPIGSTIKPFVYLVALTQPQRWN
LASLMDDGPVSMRQPDGSYWTPQNDDHRSHDPLIMVDALAHSWNLATIHLGLQVGLPRIQ
AFLKSFGIGDINPSPSLLIGAQDLAPLQLTQLYQYLAADGHALPLVAVRGVLDGNGRTIK
RYQVQGGPGEYRDAVRLTTWAMQQVAEYGTASALGNSALASLHAAGKTGTSNDMRDSWFA
GFTGEHLAVFWMGRDDNKPTTLFGASGGLRAWRELFARLPTRPLSAAPGEGLEMAWINPS
SGRRTEPQCAGAQQVPVIAGTVSADAEGCFWQGIQSLFGGSNGPPPAAAGAASTAPDLPN
GN