Protein Info for LRK53_RS14750 in Rhodanobacter sp000427505 FW510-R12
Annotation: nucleotide 5'-monophosphate nucleosidase PpnN
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 57% identical to PPNN_ECOLI: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase (ppnN) from Escherichia coli (strain K12)
KEGG orthology group: K06966, (no description) (inferred from 72% identity to xca:xccb100_1665)MetaCyc: 57% identical to nucleotide 5'-monophosphate nucleosidase (Escherichia coli K-12 substr. MG1655)
AMP nucleosidase. [EC: 3.2.2.4]; Inosinate nucleosidase. [EC: 3.2.2.4, 3.2.2.12]; Pyrimidine-5'-nucleotide nucleosidase. [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]; 3.2.2.- [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]
Predicted SEED Role
"Decarboxylase family protein"
MetaCyc Pathways
- adenosine nucleotides degradation III (1/1 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.2.2.4
Use Curated BLAST to search for 3.2.2.10 or 3.2.2.12 or 3.2.2.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (466 amino acids)
>LRK53_RS14750 nucleotide 5'-monophosphate nucleosidase PpnN (Rhodanobacter sp000427505 FW510-R12) MNTRYADPSGTTSTVSARISPIGGLDVLSRNEVARLRGASGSGLHVLLRRCALAVLTSGN VSDDPRAILEQYPDFDIQVLQQDRGMKIELSNAPAQAFVDGQIIRGINELLVAVVRDIVY VSTQLEQIGFDLDASAALTQSVFEILRNARILKPQVDPNLVVCWGGHSISREEYDYTKLV GYQLGLRGLDICTGCGPGAMKGPMKGATISHAKQRRRHNRYIGITEPGIIAAESPNPIVN HLVIMPDIEKRLEAFVRLGHGIIVFPGGVGTAEEILYLLGILLHPDNASTPFPLIFTGPR QSAAYFEQIDRFLRLSLGDGVARHYQIVVDDPETVARMMIKGLEKVRHNRLDTKDAFFFN WALRIPLEFQQPFRPTHEAMRALQIHHERPRHELAADLRRAFSGIVAGNVKEEGIQAIEQ HGPFAIDGDADIMRALDKLLQAFVDQHRMKLPGGTAYVPCYRVLSA