Protein Info for LRK53_RS14410 in Rhodanobacter sp000427505 FW510-R12

Annotation: secretion protein HlyD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 signal peptide" amino acids 1 to 15 (15 residues), see Phobius details PF13533: Biotin_lipoyl_2" amino acids 38 to 85 (48 residues), 26.1 bits, see alignment 8.9e-10 PF16576: HlyD_D23" amino acids 182 to 311 (130 residues), 42.8 bits, see alignment E=5.8e-15 PF13437: HlyD_3" amino acids 199 to 282 (84 residues), 39 bits, see alignment E=1.8e-13

Best Hits

Swiss-Prot: 49% identical to Y2891_YERE8: UPF0194 membrane protein YE2891 (YE2891) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K01993, HlyD family secretion protein (inferred from 71% identity to adk:Alide2_1724)

Predicted SEED Role

"Predicted membrane fusion protein (MFP) component of efflux pump, membrane anchor protein YbhG"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (330 amino acids)

>LRK53_RS14410 secretion protein HlyD (Rhodanobacter sp000427505 FW510-R12)
MIAVVVIAIGGALAFWRLHDHGDQNALRLYGNVDIREVELAFRQPGRLTHMAFDEGDAVT
TGAVMAQLDATPYREKLAAAEAELQAAQAQLDKLHSGNRPQEIAQAREQVRQAQASANEA
ARNFERQNTLLASGASSQRLVDAARAARDQSAANLAAAQASLSLATAGFRREDIAAGQAR
VAAAEAALTQARTAVADTTLIAPSDGIVIARVREPGSMVANSTPVYSLSLRDPVYLRAYV
DEAELGRIAPGTKVRIHTDSSTTVYHGQIGFISPRAEFTPKTVETTDLRTDLVYRLRVVV
SDADQGLRQGMPVTVDVDTAGAAGKPARGS