Protein Info for LRK53_RS13600 in Rhodanobacter sp000427505 FW510-R12

Annotation: Ppx/GppA family phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 505 transmembrane" amino acids 273 to 290 (18 residues), see Phobius details PF02541: Ppx-GppA" amino acids 28 to 311 (284 residues), 211.7 bits, see alignment E=1.6e-66 PF21447: Ppx-GppA_III" amino acids 318 to 490 (173 residues), 167 bits, see alignment E=3.8e-53

Best Hits

Swiss-Prot: 45% identical to PPX_PSEAE: Exopolyphosphatase (ppx) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 60% identity to xal:XALc_0572)

Predicted SEED Role

"Exopolyphosphatase (EC 3.6.1.11)" in subsystem Phosphate metabolism (EC 3.6.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (505 amino acids)

>LRK53_RS13600 Ppx/GppA family phosphatase (Rhodanobacter sp000427505 FW510-R12)
MSPAEQTPIRDGELLAAVDMGSNSFHLVVARMEHGEPRVIDRLRDTVRMAAGLRADGTLD
AERRARALACLARFGQRIAGLPSMRVRAVATNTVRRLASPQTFLTAAEAALGHPIEVVSG
REEGRLIFLGAAHDLPASREPRLVIDVGGGSTEFIIGRGLAPLHTESVQAGCIASTLRFF
PGGKLTRKRWQRARSEIGVLLQQFAEDYRESGWQDVYGSSGTAKSIGAVVQAMKFSDDGI
TPTSLAALADALVAQGQIGTLKLPGLVEERAPVIAGGVVIFEAAFAALGIERLRVCESSM
REGLLWDLLGRAGGSDPRTASIDALATRYGVDRAQARRVESTALMLFDQVARAWKLDADA
REWLSWASRVHEIGLAIAHSQHHHHGAYILRHADLAGFSRQEQQLLAAVVEMHRRKPDKA
AAAALPQRYRQLARYTTALLRLAVLFRRARRAESLPQMKLAATSKSLRLTLPLAWFEQHP
LSEADLQQEQAPMADLGIKLDISSS