Protein Info for LRK53_RS13350 in Rhodanobacter sp000427505 FW510-R12

Annotation: Glu/Leu/Phe/Val dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 PF02812: ELFV_dehydrog_N" amino acids 11 to 130 (120 residues), 117.8 bits, see alignment E=2.6e-38 PF00208: ELFV_dehydrog" amino acids 217 to 299 (83 residues), 47.9 bits, see alignment E=1.5e-16

Best Hits

Swiss-Prot: 56% identical to DHLE_GEOSE: Leucine dehydrogenase (ldh) from Geobacillus stearothermophilus

KEGG orthology group: K00263, leucine dehydrogenase [EC: 1.4.1.9] (inferred from 68% identity to xal:XALc_2523)

Predicted SEED Role

"Leucine dehydrogenase (EC 1.4.1.9)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Degradation and HMG-CoA Metabolism (EC 1.4.1.9)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (367 amino acids)

>LRK53_RS13350 Glu/Leu/Phe/Val dehydrogenase (Rhodanobacter sp000427505 FW510-R12)
MIFETIANTGHEEVVFCHNKDAGLKAIIAIHNTVLGPALGGLRMWPYKTEQEAVNDVLRL
SRGMTFKNAVAGLNLGGGKAVIIGDPSKDKSEALFRAFGRFVNSLNGRYITAEDVGIDVN
DMEYVFRETEYVTGVHQVHGGSGDPSPFTAYGSLQGLMAALQFKHGNEDVGKYSYAVQGA
GHVGGEFIKLLREQGAKVFVTDINKEAVQRCVDELGCEAVGLDEIYDVDADVYSPCALGG
TLNEQTIDRIKAKIICGPANNQLATDAIGDELNRRGVLYSPDYAVNAGGVMNVSLEIDGY
NRERAMRMMRTIYYNLGRIFEISKTENIPTYKAADRLAEERISAIGKLKLSHMGNGGTRF
QGRMRGQ