Protein Info for LRK53_RS12675 in Rhodanobacter sp000427505 FW510-R12

Annotation: DHA2 family efflux MFS transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 520 transmembrane" amino acids 22 to 43 (22 residues), see Phobius details amino acids 63 to 83 (21 residues), see Phobius details amino acids 92 to 114 (23 residues), see Phobius details amino acids 120 to 139 (20 residues), see Phobius details amino acids 149 to 171 (23 residues), see Phobius details amino acids 177 to 199 (23 residues), see Phobius details amino acids 211 to 230 (20 residues), see Phobius details amino acids 242 to 261 (20 residues), see Phobius details amino acids 282 to 305 (24 residues), see Phobius details amino acids 312 to 334 (23 residues), see Phobius details amino acids 343 to 362 (20 residues), see Phobius details amino acids 368 to 390 (23 residues), see Phobius details amino acids 484 to 506 (23 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 24 to 507 (484 residues), 504.4 bits, see alignment E=1.7e-155 PF07690: MFS_1" amino acids 29 to 422 (394 residues), 188.5 bits, see alignment E=8.4e-60

Best Hits

Swiss-Prot: 50% identical to EMRB_ECOLI: Multidrug export protein EmrB (emrB) from Escherichia coli (strain K12)

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 54% identity to bur:Bcep18194_A3229)

MetaCyc: 50% identical to multidrug efflux pump membrane subunit EmrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (520 amino acids)

>LRK53_RS12675 DHA2 family efflux MFS transporter permease subunit (Rhodanobacter sp000427505 FW510-R12)
MAATPNDAAPDAPLQPLHGGPLALLTIAVAFSTFMEVLDMTIVNVSVPHIAGSLGVSPSE
GTWTISSYALASAIMQPLTGWLARRFGEVKTFVGSVLLFVIFSMLCGLATSMPVLVIGRL
LQGAGSGPMVALSLTLLLSNYPKEKQGIALALWAMTVVVAPIFGPILGGWLTDNFSWPWI
FFINLPVGLAAAVVTWGLLRKRETKTYKAPIDMIGLALLVVGVGALQFMLDNGNDHDWFA
SPMILVLGLTALVCITFLIVWELHAKHPVVDLSLFRQRNFTVGVVSLSLGMFAFFGINVV
FPLWLQITLGYTATWAGLATAPVGVLAFLIAPVLGKNMHRLDLRAVVTFSFLVFAGTSYW
FSTFDSSASFAALVVPRFVMGLAIPCFFIPLNQVYLSGLPVDQIASASGLSNFCRTIGSS
VSTAVMVTLWQHRGDSHHASLTEYVSPGHPATTGFLGQLTHGGLSHTQSLGLIDRLLTRE
ALTLAVNDVFWASAILFMLLIPLLWFAKPPFGSTGGAVGH