Protein Info for LRK53_RS12415 in Rhodanobacter sp000427505 FW510-R12

Annotation: NAD-dependent protein deacetylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 277 PF02146: SIR2" amino acids 19 to 221 (203 residues), 155.8 bits, see alignment E=5.9e-50

Best Hits

Swiss-Prot: 72% identical to NPD_BORBR: NAD-dependent protein deacetylase (cobB) from Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)

KEGG orthology group: None (inferred from 70% identity to bpt:Bpet3406)

MetaCyc: 47% identical to NAD-dependent lipoamidase SIRT4 (Homo sapiens)
RXN-22964 [EC: 2.3.1.313]

Predicted SEED Role

"NAD-dependent protein deacetylase of SIR2 family" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate or Redox-dependent regulation of nucleus processes

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.313

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (277 amino acids)

>LRK53_RS12415 NAD-dependent protein deacetylase (Rhodanobacter sp000427505 FW510-R12)
MTPLQQFLEAHPRLFVLTGAGCSTASGIPDYRDRDGQWKRPPPVNYSAFMHEPATRQRYW
ARSMVGWRRFGSAQPNAAHHALATLEQRGQVELLVTQNVDRLHQRAGSERVLDLHGRLDE
VRCMGCDWRGARHAFQQRLLERNPAWAQLDAVDAPDGDAELEGLDFASFEVPPCPRCGGI
VKPDVVFFGEAVPRERVETATRAWQAADAVLVVGSSLMVYSGYRFVAAAAQAGKPVAAVT
LGRTRADALLTLKVDAPCQEALAFLRASEPAAAVPLP