Protein Info for LRK53_RS12345 in Rhodanobacter sp000427505 FW510-R12

Annotation: lipoyl synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16881: LIAS_N
  • amino acids 29 to 75 (47 residues), 28.1 bits, 2.4e-10
• TIGR00510: lipoyl synthase
  • amino acids 33 to 321 (289 residues), 370 bits, E=4.7e-115
• PF04055: Radical_SAM
  • amino acids 92 to 255 (164 residues), 65.5 bits, E=6.9e-22

Best Hits

Swiss-Prot: 63% identical to LIPA_PSEMY: Lipoyl synthase (lipA) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K03644, lipoic acid synthetase [EC: 2.8.1.8] (inferred from 79% identity to gpb:HDN1F_14530)

MetaCyc: 50% identical to lipoyl synthase (lipoic acid synthetase) (Bacillus subtilis subtilis 168)
Lipoyl synthase. [EC: 2.8.1.8]

Predicted SEED Role

"Lipoate synthase" in subsystem Lipoic acid metabolism

MetaCyc Pathways

• lipoate biosynthesis and incorporation I (2/2 steps found)
• lipoate biosynthesis and incorporation III (Bacillus) (2/3 steps found)
• lipoate biosynthesis and incorporation V (mammals) (2/3 steps found)
• lipoate biosynthesis and incorporation IV (yeast) (4/7 steps found)
• lipoate biosynthesis and incorporation II (1/3 steps found)

KEGG Metabolic Maps

• Lipoic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.8

Use Curated BLAST to search for 2.8.1.8

Protein Sequence (325 amino acids)

>LRK53_RS12345 lipoyl synthase (Rhodanobacter sp000427505 FW510-R12)
MSSSHSVPDGSKFTSPQGTRAVKGGQKPNTHSHEPDVGRKPPWLRVKLPSGAHYEEVADI
VRTHQLNTVCAESKCPNIAECWGRGTATLMLMGSVCTRACKFCSVSTGNPQGWLDPLEPL
QVADAVALMGLKYVVLTSVDRDDLADGGAAHYAACVRAIHQRAPQAAVEALTPDFAGNHA
AVVTVLDAGLVTYAQNIETVERLTHAVRDPRAGYAQTLGVLAFAKRHAPQTLTKTSLMLG
LGETDAEIERTLDDIRGANVDLVTLGQYMRPSPHHLPVQRFVTPDEFRHYRELALGKGFV
EAVAGPLVRSSYRAERVLEHNNLGL