Protein Info for LRK53_RS12010 in Rhodanobacter sp000427505 FW510-R12

Annotation: bifunctional diguanylate cyclase/phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 853 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 416 to 576 (161 residues), 155.3 bits, see alignment E=6.2e-50 PF00990: GGDEF" amino acids 417 to 572 (156 residues), 167.3 bits, see alignment E=2.5e-53 PF00563: EAL" amino acids 594 to 828 (235 residues), 249 bits, see alignment E=4.2e-78

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (853 amino acids)

>LRK53_RS12010 bifunctional diguanylate cyclase/phosphodiesterase (Rhodanobacter sp000427505 FW510-R12)
MNSGILVVAAQRELRRSLFDALDQAGYPQIHSARDVSHAAILLEGRAALPPLQLMVVVLA
GDEVQARIACEQLRRLPGGADTPLIVVLGDDATLGPSDLPTGVADWLSAAQIGSELVPRW
RRWQARNGQRRPPAAASDGDAHEDYRYVFDESDSEWLIADAQTGRLLEVSPTVARHSRMH
ASQWEGLPLAEVLRFEGIAIDQVLAEANRGWYPCQRKSAIGADTGQASVRRIRHAGREAL
ALMFRSDRADLRAEAALSLLSRIFASTSGVDAQNAAGRLLFDELGLDYLAVWSARQDGDA
PVQMLRLWNGDEPAWPPPTLQSSLQLVLGGKPILYRTDAKRLASVDPLLQQLDLAGFAGL
PLYDERHTVLGAMLAGSRRGFGKMGIIEPVLRCAAARFAQMLELGRTREQGRAEGLVDAL
TGLPNRLLFNDRLDTIIREATRNGECFAVLFVDLDRFKAINDTYGHAAGDQVLRMVTQRL
CGSIRASDTVARYAGDEFTVVLRHIVKNDDVLRVAEKIVQVMETPLFLEDGTELQVTASM
GLSFFPDDAGDAQTLLKHADEAMYAAKHQGRNTFQIYEVSPEYAQQHGMALKTRLRHAEG
NGELRVVYQPQVNTETEDIMGMEALVRWEHPELGMISPAVFIPLAEESGLIVSIGEWVMR
TACRQAKEWEQLYGLRLRLGVNLSAVQLMEPQLLETVASVLRETGLDPTLLEMEVTESIS
IKEAPNLVENLHALHRLGCHIAIDDFGTGAASLDYLRRLPADRIKIDQSFVRNIGVDPDD
EAIVRATIEMAHRLKRAVVAEGVEIEQHLQFLRQHGCDELQGYLFCRPLPPASFDKLLAE
RQRLLKARVAEPA