Protein Info for LRK53_RS11925 in Rhodanobacter sp000427505 FW510-R12

Annotation: hypoxanthine-guanine phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 36% identical to HPRT_ENTFA: Hypoxanthine-guanine phosphoribosyltransferase (hpt) from Enterococcus faecalis (strain ATCC 700802 / V583)

KEGG orthology group: K00760, hypoxanthine phosphoribosyltransferase [EC: 2.4.2.8] (inferred from 54% identity to xcv:XCV1387)

MetaCyc: 30% identical to hypoxanthine phosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Hypoxanthine phosphoribosyltransferase. [EC: 2.4.2.8]; 2.4.2.8 [EC: 2.4.2.8]

Predicted SEED Role

"Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8)" in subsystem Purine conversions (EC 2.4.2.8)

MetaCyc Pathways

superpathway of purine nucleotide salvage (14/14 steps found)
guanine and guanosine salvage I (2/2 steps found)
guanine and guanosine salvage II (2/2 steps found)
xanthine and xanthosine salvage (2/2 steps found)
adenine and adenosine salvage III (3/4 steps found)
adenine salvage (2/3 steps found)
superpathway of guanine and guanosine salvage (2/3 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (183 amino acids)

>LRK53_RS11925 hypoxanthine-guanine phosphoribosyltransferase (Rhodanobacter sp000427505 FW510-R12)
MNTPTPSLADALAQAELLHDRATLESVIADMGRRIDTALDGERAVFLTVMNGALIFAGQL
ALAIRTDLEFDYVHATRYRGATSGSELHWLREPAVPLAGRTVLLVDDILDEGHTLKAVRD
DCYRRGANKVLIVSLCTKRHDRLVEGIASDFNGVELPDRYVFGYGMDYYEQGRNLPGIYA
LKD