Protein Info for LRK53_RS11600 in Rhodanobacter sp000427505 FW510-R12

Annotation: glutamine--fructose-6-phosphate transaminase (isomerizing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 609 (608 residues), 811.9 bits, E=1.7e-248
• PF13522: GATase_6
  • amino acids 66 to 181 (116 residues), 69 bits, E=6.2e-23
• PF13537: GATase_7
  • amino acids 79 to 196 (118 residues), 53 bits, E=5.1e-18
• PF01380: SIS
  • amino acids 295 to 415 (121 residues), 99.5 bits, E=2e-32
  • amino acids 461 to 591 (131 residues), 80.1 bits, E=2e-26

Best Hits

Swiss-Prot: 59% identical to GLMS_PSEPK: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 63% identity to mca:MCA0015)

MetaCyc: 58% identical to L-glutamine--D-fructose-6-phosphate aminotransferase (Escherichia coli K-12 substr. MG1655)
Glutamine--fructose-6-phosphate transaminase (isomerizing). [EC: 2.6.1.16]

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (11/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.16

Use Curated BLAST to search for 2.6.1.16

Protein Sequence (609 amino acids)

>LRK53_RS11600 glutamine--fructose-6-phosphate transaminase (isomerizing) (Rhodanobacter sp000427505 FW510-R12)
MCGIVAAAAQRDVAPLLIAGLKALEYRGYDSSGVAVLDGGAIHRVRAKGKVREMEALYLA
DPLPGGTGIAHTRWATHGVPNEANAHPHIAGRVSIVHNGIIENHARLRAELEAAGHVFTS
DTDTEVMAALIDRHLDQGLSLREAVLATVRELEGAYAIAVLSRDEPGRVVGARRGAPLLV
GVGIGENFLGSDAQALVQVTHKMLYLDEGDVVEITRDSVQVFGLDGAPVERAVHESELST
DAVERGEYRHYMQKEIFEQPQAVANTLEARLGPHGVLPNIFGIDSDALLQATRGLHIVAC
GTSYHAGLVAKYWIEEYARLPVSVEVASEYRYRETVVPEGTLFVAISQSGETADTLAAMR
ESRRRGYLGTLAICNVPESSAVREADLKLMTRAGPEIGVASTKAFTTQLAGLALLTLELA
RRHGLDADRYAALCAELQHLPRAIEETLQMEPAIIALAGEFIHRQHALFLGRGVQYPVAM
EGALKLKEISYIHAEAYAAGELKHGPLALVDEHMPVIAVAPNGPLLDKLKSNLQEVRARG
GQLIVFADGAAGMDDMAGTGRMLRVASGGNFIAPAVFTIPLQLLAYHVAVLRGTDVDQPR
NLAKSVTVE