Protein Info for LRK53_RS11590 in Rhodanobacter sp000427505 FW510-R12

Annotation: phosphoketolase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 PF09364: XFP_N" amino acids 12 to 373 (362 residues), 580.2 bits, see alignment E=2.2e-178 PF03894: XFP" amino acids 403 to 579 (177 residues), 294.5 bits, see alignment E=4e-92 PF09363: XFP_C" amino acids 590 to 791 (202 residues), 315.6 bits, see alignment E=1.8e-98

Best Hits

Swiss-Prot: 74% identical to PHK_RHOBA: Probable phosphoketolase (RB4903) from Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)

KEGG orthology group: K01621, phosphoketolase [EC: 4.1.2.9] (inferred from 72% identity to dba:Dbac_1358)

Predicted SEED Role

"Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) @ Fructose-6-phosphate phosphoketolase (EC 4.1.2.22)" (EC 4.1.2.22, EC 4.1.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.2.22, 4.1.2.9

Use Curated BLAST to search for 4.1.2.22 or 4.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (800 amino acids)

>LRK53_RS11590 phosphoketolase family protein (Rhodanobacter sp000427505 FW510-R12)
MTTDAFAHPPLDSDQLYGIDAWWRAANYLSVGQIYLRANPLLKEPLALAHIKHMLLGHWG
TTPGQNFIYAHLNRAIRQYDLDMFYISGPGHGGPAVVANAYLDGTYSEVYPDISQDEAGL
QRLFKQFSFPGGIPSHASPECPGSLHEGGELGYSLSHAFGAVFDNPGLIAACVVGDGEAE
TGPLATAWHSNKFLDPVGDGAVLPILHLNGYKIANPAVLARIGRDELEPLLRGYGWTPFF
VSGSEPAAMHQAMAAALDAVVAQIRQIQRDAREHGVTARPRWPVIVLDSPKGWTGPKLVD
GKPNEGTFRSHQVPLSISASSPPEHLQQLEAWLRSYRPEELFDAHGRLRPEIAALAPAGT
RRMSANPHTNGGLLLRDLRLPDFRDYALAVPQPGVRGSGDTRALGPFLRDVIRLNESSRN
FRVFGPDETVSNGLNAVFEATNRQWEAPLKPNDEFLATQGRVVEMLSEHQCEGWLEGYLL
TGRHGLFNCYEAFIHIVDSMFNQHAKWLKMTAAIPWRRPLASLNYLLASHVWRQDHNGFT
HQDPGFIDHVVNKKASIVRVYLPPDANCLLSVMDHCLRSRHYVNVVIAGKHPAPQWLAMD
AAQRHCSLGIGVWPWASSDQHGEPDVVMACAGDVPTLETLAAVTILRRYLPELKLRVVNV
VDLMKLQSPSEHPHGLGDDDFDQLFTRDKPVIFAYHGYPSLIHRLTYRRTNHDNLHVHGY
QEEGTITTAFDMTVLNALDRFHLVIDVIDRVPQAGERGIYLKQKLLDKLLEHKQYIDDNG
EDLPEIRNWVWGAQDAGVLS