Protein Info for LRK53_RS11555 in Rhodanobacter sp000427505 FW510-R12
Annotation: EF-P lysine aminoacylase EpmA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K04568, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 74% identity to xca:xccb100_2689)Predicted SEED Role
"Translation elongation factor P Lys34:lysine transferase"
MetaCyc Pathways
- tRNA charging (21/21 steps found)
- myxochelin A and B biosynthesis (1/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.1.1.6
Use Curated BLAST to search for 6.1.1.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (318 amino acids)
>LRK53_RS11555 EF-P lysine aminoacylase EpmA (Rhodanobacter sp000427505 FW510-R12) MGRVPLAALQLRARLYALVRAYFAEQRVLEVETPMLSAAGNTEPNIEGFSTMFSGHVDAG ARERWLRTSPEHPLKRLLAAGIGDCYELGRVFRNGEAGGRHNPEFTMLEWYRVGWDHRQL MQETVALVEAALAMVGRRAELVVTSYRQLFLDELGIDPAHAAIDTLRAPLAESGIDPAGL GHDDWLDLLITHKLQPAFPRDRITVIHDYPASQCALARIRPGEPPLAERFELYLGRYELA NGYHELNDAAEQRARFERDNARRRARGLREVPLDERLLGVLDALPDCAGVALGVERLLMC LADTDAIADVLAFAFSEA