Protein Info for LRK53_RS11530 in Rhodanobacter sp000427505 FW510-R12

Annotation: exodeoxyribonuclease I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 PF00929: RNase_T" amino acids 6 to 186 (181 residues), 77.6 bits, see alignment E=1.7e-25 PF08411: Exonuc_X-T_C" amino acids 205 to 473 (269 residues), 302.4 bits, see alignment E=2.4e-94

Best Hits

Swiss-Prot: 45% identical to EX1_ECOLI: Exodeoxyribonuclease I (sbcB) from Escherichia coli (strain K12)

KEGG orthology group: K01141, exodeoxyribonuclease I [EC: 3.1.11.1] (inferred from 54% identity to mmt:Metme_2322)

MetaCyc: 45% identical to exodeoxyribonuclease I (Escherichia coli K-12 substr. MG1655)
Exodeoxyribonuclease I. [EC: 3.1.11.1]

Predicted SEED Role

"Exodeoxyribonuclease I (EC 3.1.11.1)" in subsystem DNA Repair Base Excision (EC 3.1.11.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (484 amino acids)

>LRK53_RS11530 exodeoxyribonuclease I (Rhodanobacter sp000427505 FW510-R12)
MQTFFWHDYETSGADPRRDRPLQFAGIRTTPELEIVGEPVMFYGQPSREMPPHPDACLIT
GITPQQAEREGLGEAEFAARVHEQLAAPGTCGVGYNSLRFDDEFTRQMLYRNFHEPYGRE
WENGNSRWDLIDLVRMCQALRPEGIVWPTREDGSPSFKLEHLASANGLTQERAHDALSDV
RALIELARLIRVRQPRLWDWYYALRRKQKVFELLDVVNMTPLLHVSSRYPASRHCLAVIA
PLAAHPSRPGEVIVYDLATDPAAWLALDEEEIADRIFTARADLPEDVERIPLRTVRANHA
PALAPLSVLQGVDLSRLQLDLARCQAHRDVLHAVDGLAEKLRRVFQRAAELPPPEDPELA
LYGGGFLPDADKRLLAQVRATPPSELGTRSFPFRDPRYPELLFRYRARNWPETLDTEEYA
RWENFRRARLTTHTPLTGLTLGDYFARLAELRHAPQAQDKLPLLDQLQAWGEQLAASIND
NSST