Protein Info for LRK53_RS10720 in Rhodanobacter sp000427505 FW510-R12

Annotation: acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 608 transmembrane" amino acids 315 to 332 (18 residues), see Phobius details PF12418: AcylCoA_DH_N" amino acids 6 to 34 (29 residues), 37.4 bits, see alignment (E = 6.2e-13) PF00441: Acyl-CoA_dh_1" amino acids 301 to 469 (169 residues), 64.8 bits, see alignment E=3e-21 PF12806: Acyl-CoA_dh_C" amino acids 489 to 604 (116 residues), 94.8 bits, see alignment E=1.3e-30

Best Hits

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 72% identity to reh:H16_A1530)

Predicted SEED Role

"3-methylmercaptopropionyl-CoA dehydrogenase (DmdC)"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.3

Use Curated BLAST to search for 1.3.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (608 amino acids)

>LRK53_RS10720 acyl-CoA dehydrogenase (Rhodanobacter sp000427505 FW510-R12)
MDSLLLSRRDVNFLLYEWLDVAALTARPRHADHSRETFDAVLDVAVRLATDRFAPHNQKA
DQCEPRFDGEHVHLIDELKPALQAFAETGLSAGSKDYALGGMQLPHVVEMAALAYLYAAN
VSTSAYPLLTMGNANLLLAHGNPAQIKAFVEPELAGRFFGTMCLSEPQAGSSLSDITTRA
EPDGDSPFGPRYRVTGNKMWISGGDHELSENIVHLVLAKIPGTDGKLIPGVKGISLLLVP
KFLVHEDGSLGERNDVELAGINHKMGYRGTVNTLLNFGEGSRHTPEGRAGAIGYLVGEAH
QGLAYMFHMMNGARIGVGLGAVALGYTGYLHALDYARHRPQGRLVGAEGKDPSSPQTRII
EHADVKRMLLAQKAYVEGGLAFNLYCARLFDEAATSDDENERANLTLLLDILTPIAKSWP
SQWCLAANDLAIQVHGGYGYTREYKVEQFYRDNRLNSIHEGTHGIQALDLLGRKVVMKDG
AAFRLFSARVAGSIDRAGKQDSLRTHAENLEARVHRLAEVTRQLWAAGNPLLTLANASVY
LEAFGHIAIAWIWLEQALTAVGSAGNFYEGKRCAADYFFRWELPKVDAQLNLLASMDRTT
LDMQDAWF