Protein Info for LRK53_RS10195 in Rhodanobacter sp000427505 FW510-R12

Annotation: polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 703 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 10 to 692 (683 residues), 1055 bits, see alignment E=0 PF01138: RNase_PH" amino acids 14 to 143 (130 residues), 90 bits, see alignment E=5.2e-29 amino acids 324 to 457 (134 residues), 87.1 bits, see alignment E=4.3e-28 PF03725: RNase_PH_C" amino acids 146 to 210 (65 residues), 50.7 bits, see alignment E=4.4e-17 amino acids 461 to 529 (69 residues), 31 bits, see alignment E=5.8e-11 PF03726: PNPase" amino acids 241 to 321 (81 residues), 43.2 bits, see alignment E=1.3e-14 PF00013: KH_1" amino acids 557 to 615 (59 residues), 40.9 bits, see alignment 4.2e-14 PF23459: S1_RRP5" amino acids 620 to 683 (64 residues), 28.2 bits, see alignment E=7.1e-10 PF00575: S1" amino acids 620 to 691 (72 residues), 67.9 bits, see alignment E=2.6e-22

Best Hits

Swiss-Prot: 68% identical to PNP_XANCB: Polyribonucleotide nucleotidyltransferase (pnp) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 68% identity to xcb:XC_1609)

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.8

Use Curated BLAST to search for 2.7.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (703 amino acids)

>LRK53_RS10195 polyribonucleotide nucleotidyltransferase (Rhodanobacter sp000427505 FW510-R12)
MKKTLKALAFGKHELIIETGAVARQASAAVTVRLDDTVVLVTAVADKRLSPGRDFFPLTV
NYQEKFYAAGRIPGGFFKREGRLTEKETLVSRLIDRPIRPLFPKEFLHEVQVVATVMSLN
PEVDSDIPALIGASAALALSGVPFAGPIGAARVGYCGGEFVLNPTQTELRTSELDLVVAG
TSKAVLMVESEASLLSEGIMLSAVMFGHRAMQPVIAAIRELVAEAGVTPWSWTVPARNEA
LTDRVRSLVGLSLVEAYAIADKGERQAAVRAARDAMLEVIDQERESHGWTSQQAIAAFED
LEHHTVRQLVLESRKRIDGRRLDEVRPIHIDVHNLPRPHGSAMFTRGETQAIVTATLGTG
RDAQLIDAAEGESKELFMLHYNFPPYCVGEAGFMGGPKRREIGHGRLAKRGLKAVLPSFD
DFPYVLRVVSEITESNGSSSMASVCGGSLALMDAGVPLKAPVAGIAMGLVKEGFRHAVLT
DILGDEDHLGDMDFKVAGTRGGVSALQMDIKIDGITEEIMREALAQARQGRLHILDEMDK
ALDRPRVELSEYAPRLVTFRISPDKIRDVIGKGGVTIRSITEETGVTLDIRDDGTVVIAS
RDAAATARARARVEQITTDVEPGSVYEGRVAKIMDFGAFVTILPGKDGLVHISQIAEDRV
ECVADHLSEGDLVRVKVLEVDRQGRIRLSMKALAEPATVPDVA