Protein Info for LRK53_RS09915 in Rhodanobacter sp000427505 FW510-R12

Annotation: error-prone DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1026 TIGR00594: DNA polymerase III, alpha subunit" amino acids 5 to 970 (966 residues), 516.3 bits, see alignment E=9.2e-159 PF02811: PHP" amino acids 6 to 99 (94 residues), 68.1 bits, see alignment E=3e-22 PF07733: DNA_pol3_alpha" amino acids 268 to 523 (256 residues), 281.7 bits, see alignment E=1.6e-87 PF17657: DNA_pol3_finger" amino acids 526 to 692 (167 residues), 188 bits, see alignment E=2.1e-59 PF14579: HHH_6" amino acids 766 to 857 (92 residues), 67.2 bits, see alignment E=3.1e-22 PF01336: tRNA_anti-codon" amino acids 936 to 1005 (70 residues), 30.3 bits, see alignment 8.4e-11

Best Hits

Swiss-Prot: 64% identical to DNAE2_PSEMY: Error-prone DNA polymerase (dnaE2) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 65% identity to psa:PST_2347)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1026 amino acids)

>LRK53_RS09915 error-prone DNA polymerase (Rhodanobacter sp000427505 FW510-R12)
MSDYAELHCLSDFSFGRGASNAAELFERARACGYSALAITDECSLAGIVRAYQASNETGL
KLIVGAEFQLEDGPKLVLLCENRQGYAELCRLITRGRRASGKGTYRLTCADLESGMPGTL
ALWMPGLQADTAHGRWIRATFGDRAWLAVELHRGPDDVGRLCELQALGHALDLPLAASGD
VHMHVRRRLALQHTLTAIRHRVPIAEAGALIFRNGERHLRRRDVLAGIYPAALMQESVRI
AERCTFHMGELKYDYPTELVPDGHTPASWLRQLAEEGIRWRWPQGASAKVRKLIDDELAL
IASKQYEAFFLTVHDIVRFARGEGILCQGRGSAANSAVCFALGVTEVDPEVNHLLVARFI
SEDRDEPPDIDVDFEHERREEVIQYIYGKYGRERAALAATVICYRGRSAVRDVAKALGLP
LDQIDRLSDVFARGWGDSKADERLREQGFDPDSPIIRRVLKLTYELLGMPRHLSQHVGGF
VISDTSLSEMVPVENAAMPDRTVIQWEKDDLDYMRMLKVDCLALGMLTCLRKCFGLLESG
HGVAKTIATIEPGDRATYEMIQRADTVGVFQIESRAQMAMLPRHRPANFYDLVIQVAIVR
PGPIQGDMVHPYLRRRNKEEVVDYPSPQFKDVLERTLGVPLFQEQVMKLAIVAADFTDSE
ADKLRRSMAAWKRHGGLEPHRDKLMQGMLKNGYTAAFAARIFEQIKGFGSYGFPESHAAS
FANLVYASCWLKCHYPAAFACALLNAQPMGFYGPSQIVQDVRRHRVAVRPVDVRFSDWDC
TLEPDPRGHADARAIRLGLRMVRGCSEAAALRLMAARGRQPFADVTDLCSRAGLDRRHQE
LLADAAALRGLAGHRHRARWAVAGVEPQLPLFGSESPGERAVALPLPTQAEDTLADYARV
GLSLGAHPLRQIRARLNAAHCMDGKTLRRQPHHSRVRVAGLVTSRQQPQTASGIIFVTLE
DEHGLINVIVRRQVAEAQRRALLQARLLAVEGQWENVDRVSHLIAHRLRDLTPLLGALDA
RSRDFH