Protein Info for LRK53_RS09610 in Rhodanobacter sp000427505 FW510-R12

Annotation: aminomethyl-transferring glycine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 962 transmembrane" amino acids 332 to 352 (21 residues), see Phobius details amino acids 748 to 768 (21 residues), see Phobius details PF02347: GDC-P" amino acids 19 to 439 (421 residues), 601.3 bits, see alignment E=1.1e-184 amino acids 448 to 730 (283 residues), 44.9 bits, see alignment E=7.8e-16 TIGR00461: glycine dehydrogenase" amino acids 20 to 950 (931 residues), 1475.7 bits, see alignment E=0 PF21478: GcvP2_C" amino acids 778 to 899 (122 residues), 198.9 bits, see alignment E=2.2e-63

Best Hits

Swiss-Prot: 67% identical to GCSP1_PSEAE: Glycine dehydrogenase (decarboxylating) 1 (gcvP1) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (962 amino acids)

>LRK53_RS09610 aminomethyl-transferring glycine dehydrogenase (Rhodanobacter sp000427505 FW510-R12)
MSHNPPSLRDLEHHGAFIERHIGPDDAEIAQMLHVIGHDSLESMTDAIVPGAIKSAAPLA
LPPAITEEDALAKIRAIASKNQVFRSFIGQGYYGTHTPNVILRNILENPAWYTAYTPYQA
EISQGRMEALINFQTMCADLTGMEIANASLLDEATAAAEAMTLAKRSCKSKSNVFFVSNG
VHPQTLEVVRTRAEPLGIELVVGADADAAGTEAFGVLLQYPDTFGGIHDYRAIADAVHAR
GGLVAVATDLLALTLLAAPGEWGADIVVGNSQRFGVPFGFGGPHAAFMACRDAYKRSMPG
RLIGVSIDAEGKPAYRLTLQTREQHIRREKATSNICTAQVLLAVMASMYAVYHGPKGLVR
IARRTHRLAAILAAALRQASVAVGKEFFDTLHVTGVDAAALHAKAAAARINLRRIDGGSV
GVSLDESTTRADVIALAALFGGNIADIDALDAATADALPAALARKSAFLTHPVFNTHHSE
HELLRYMRALADKDLAMDRTMIPLGSCTMKLNATAEMIPITWPEFANIHPLAPAAQAQGY
KELIDGLEAMLVECTGYDAVSLQPNSGAQGEYTGLLAIRAYHRSRGEAHRDICLIPESAH
GTNPASAHLCGMTVVVTKCDANGNVDVEDIRAQAGKYSDRLAALMITYPSTHGVFEEDIV
AICDIVHQHGGQVYTDGANMNALVGVAKPGKWGSDVSHLNLHKTFCIPHGGGGPGVGPCA
VKSHLAPFLPKAFGADGVRTHGSGSNAMVSAASFGSASILPISWMYIAMMGAEGLRKATQ
VALLNANYIARRLAPHYPTLYTGRNGLVAHECILDLRPLKDATGIGAEDVAKRLIDFGFH
APTLSFPVSGTLMVEPTESESRYELDRFIDAMIQIRDEIRAIEEGKLEREDNPLKNAPHT
ATMVSGSEWTHAYPRELAVFPLASLKLQKYWPPVARVDNVYGDKHIQCACIPVDAYKEEA
EA