Protein Info for LRK53_RS09185 in Rhodanobacter sp000427505 FW510-R12

Annotation: assimilatory sulfite reductase (NADPH) hemoprotein subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02041: sulfite reductase (NADPH) hemoprotein, beta-component
  • amino acids 9 to 551 (543 residues), 828.9 bits, E=8.6e-254
• PF03460: NIR_SIR_ferr
  • amino acids 69 to 129 (61 residues), 57.2 bits, E=1.1e-19
  • amino acids 344 to 409 (66 residues), 40.6 bits, E=1.7e-14
• PF01077: NIR_SIR
  • amino acids 163 to 320 (158 residues), 137.5 bits, E=2.7e-44

Best Hits

Swiss-Prot: 64% identical to CYSI_MYXXD: Sulfite reductase [NADPH] hemoprotein beta-component (cysI) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 68% identity to put:PT7_0173)

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Protein Sequence (566 amino acids)

>LRK53_RS09185 assimilatory sulfite reductase (NADPH) hemoprotein subunit (Rhodanobacter sp000427505 FW510-R12)
MSTKLSHLEHIKSASRYLRGTIAEGLADPVTGAIGDDDNKLLKFHGSYQQDDRDLREERR
KQKLEPAYAFMLRARLPSGVVTPAQWLVFDRIAREHASGTLRITTRQTFQWHGIIKHKLK
PTIAAIHEALATTIAACGDVVRNVVSTANPVESAAHAEAYAWATRLSTELAPKTRAYHEI
WLDGEKLHGDPVAESEPLYGATYLPRKFKIGLAIPPLNDIDVFAQDLGLIAIVEQGTLRG
FNIAVGGGMGATHGDASTYPRLANVIGFATPEQLFGVAEAVIGIQRDWGDRAERQHARLK
YTLDRHGLDAFKAELEARLGYALAAPRAYRFEHNGDRYGWVEGHDGRWHLTLHIESGRLA
DTGERRWLSGLRELAKVHAGDFRLTCNQNVIVANVAAAERERIDAIVREHGLDGYRKQSA
IRRHAIACVALPTCGLAMAESERYLPALLPKLEALLEHHGLADAPILLRLSGCPNGCSRP
YLGEIALVGRGPGRYDLRLGADFSGQRLNQVYRENVDEAAILAALDPLFARYAAERAAGE
GFGDFLLRAGILAPLPSRRIPSELVA