Protein Info for LRK53_RS08680 in Rhodanobacter sp000427505 FW510-R12

Annotation: bifunctional tetrahydrofolate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 24 to 419 (396 residues), 304.8 bits, E=6.4e-95
• PF08245: Mur_ligase_M
  • amino acids 46 to 219 (174 residues), 31.6 bits, E=1.6e-11
• PF02875: Mur_ligase_C
  • amino acids 290 to 412 (123 residues), 64.2 bits, E=2.3e-21

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of chorismate metabolism (39/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (424 amino acids)

>LRK53_RS08680 bifunctional tetrahydrofolate synthase/dihydrofolate synthase (Rhodanobacter sp000427505 FW510-R12)
MSRTLAEWLAYQERVNVHSIELGLDRVREVWRRMSAPAPAKRVITVGGTNGKGSTVALLE
AMLRAADLRVGAFTSPHLLEYNERVRIGGVDADDAALIASFERIEASRGSMPLTYFEFGA
LAALDLFARAALDVAVLEVGLGGRLDAVNIIDADVAVITTVDLDHMEWLGPDRDSIGHEK
AGIARAGRPAIVGELDPPAGLLDALAERGARVERAGIDFSIERHQDGWRWRHRDGSAMEL
PDPALAAPVQYANAAAAIAALHALDLEAFTPSSFFAAVSAGLHEVRVPARLQSIGGEPPV
IVDVGHNPQAARALAEWLDLQAPTRVHAVYGALADKDVAGVIGALGPHIGHWHLAGLDQA
TPRGMPVAALAAVLRQVLPQAPYDAHADVTAALAAARAVAQPNERILAFGSFFVASAVLA
ERIG