Protein Info for LRK53_RS07740 in Rhodanobacter sp000427505 FW510-R12

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 996 PF00133: tRNA-synt_1" amino acids 29 to 698 (670 residues), 469.9 bits, see alignment E=1.6e-144 PF09334: tRNA-synt_1g" amino acids 60 to 203 (144 residues), 38.6 bits, see alignment E=1.5e-13 amino acids 641 to 691 (51 residues), 23.3 bits, see alignment 6.7e-09 PF01406: tRNA-synt_1e" amino acids 634 to 706 (73 residues), 31.4 bits, see alignment 3.3e-11 PF08264: Anticodon_1" amino acids 743 to 898 (156 residues), 94.6 bits, see alignment E=1.4e-30 PF06827: zf-FPG_IleRS" amino acids 958 to 983 (26 residues), 30 bits, see alignment (E = 8.9e-11)

Best Hits

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (996 amino acids)

>LRK53_RS07740 isoleucine--tRNA ligase (Rhodanobacter sp000427505 FW510-R12)
MTHDYKSTINLPQTDFPMRGDLPKREPGWLAEWERVGRYAQIQRKTAHRDSVFVLHDGPP
YANGSIHIGHAVNKILKDMVVKSKLLAGQRAPYVPGWDCHGLPIEIAVEKKYGKPGEKLD
AAQFRQKCREYAQQQIDDQRAGFRRLGVLGDWEHPYRTMDFKYEADMLRALARIVSNGHV
VRGAKPVYWCFDCASALAEAEIEYGDKVSPAVDVAYDAVDPKALAAKFGVDAGDAIVAIP
IWTTTPWTLPESQAVSLGGGLEYALIEGPLRDGRRVLLVVASALVDKVRARYSLLPSGEG
ARRADEGTAGAQPPRALEPSPPTPLTREGTPLGSPTGEGSQVFGHAEGKALEGLKLHHPF
YPREVPVILGEHVSAEDGTGAVHTSPDHGVEDFVVAREYGIGLLNYIGSRGSYRADTPAA
DGLELAGMHIWKANDAIVELLRQRGVLLAFAKIEHSYPNCWRHKTPVIYRTTPQWFISME
QAQLRETALTSIKNVRWVPGWGEERIAGMVAGRPDWCISRQRTWGVPIALFVHKATQEPH
PDSVALLEQVAKKVEQGGIDAWFALDAAELLGDQASDYEKVTDVLDVWFDSGVTHYAVIG
QRPELQQGRASHYKVMYLEGSDQHRGWFQSSLLTSAAIHGRAPYNEVLTHGFAVDANGRK
MSKSLGNVVAPQKVMDTLGADVLRLWVASADYRNEMTVSDEILKRVSDAYRRIRNTARFL
LGNLDGFDPAKHLLPVEDSVLLDQWAVQQAYDVQQAVTAAYERYDYPEVVSRIQNFCTNE
LGALYLDITKDRLYTMPTDSRGRRSAQSAMYRIAEAMVRWLAPILSFTAEEIWQAMPGER
GDSVLFETWYDGLAATQASPEQRHWWADLLAIRETAARVLEGMRKAERIGAALEAKLVVH
ADVATQARYAEVADELRFFFITSEFTLAPVTPRADDAARVELDGVEAWVSAEVSGAAKCV
RCWHRRDDVGSHAEHPELCSRCVGNVEGPGENRRWF