Protein Info for LRK53_RS05035 in Rhodanobacter sp000427505 FW510-R12

Annotation: NAD+ synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 5 to 244 (240 residues), 100.2 bits, E=1.3e-32
• PF02540: NAD_synthase
  • amino acids 267 to 539 (273 residues), 234.3 bits, E=1.2e-73
• TIGR00552: NAD+ synthetase
  • amino acids 272 to 545 (274 residues), 192.8 bits, E=3.1e-61

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• NAD de novo biosynthesis I (6/6 steps found)
• NAD de novo biosynthesis II (from tryptophan) (8/9 steps found)
• NAD salvage pathway II (PNC IV cycle) (5/5 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
• NAD de novo biosynthesis III (5/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
• NAD salvage pathway V (PNC V cycle) (4/5 steps found)
• superpathway of NAD biosynthesis in eukaryotes (10/14 steps found)
• NAD salvage (plants) (6/11 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5

Protein Sequence (563 amino acids)

>LRK53_RS05035 NAD+ synthase (Rhodanobacter sp000427505 FW510-R12)
MARLRLALAQYDFPVGAVAANAAKAGDLIARARNGGAALVAFPELTLSGYPPEDLLLRPS
FLAACAGELAVLAAATDGVAALVGHPHSEGEVFNAASLLRDGRVDCTAHKQVLPNYGVFD
DKRYFRPGHESTTAVVDGVRVGFLICEDVWQPEPAAQAAAEGAELLLVINASPWDGAKQA
GREAVLQTRARETGCAIAYLNLVGGQDEVVYDGGSLLVDGDGTVAARAPAFVDALLWAEF
DPATRRWSADGWPVAADASPEATLYAVLVRGTRDYIDKNGFGGVLLGLSGGIDSALVLAL
AVDALGAERVTAVMMPTRYTSQLSLDGARAQAQGLGVDYHVIDIEPTYRSFIAALTPAFA
GKAADTTEENLQSRIRGTLLMALSNKHGRLLLATGNKSEMAVGYATLYGDMCGAYAPLKD
VYKTVVYRLARWRNGAGNRDSGFGIRPSASARDGGFSRIPNPESPIPDEVIARPPSAELR
DNQTDQDSLPSYEVLDAILARFIEGEQSQAEIVAAGFAADVVHRVVRLVLVNEFKRRQAA
PGPRVTTRAFGRERRYPITSGWR