Protein Info for LRK53_RS04490 in Rhodanobacter sp000427505 FW510-R12

Annotation: adenylyl-sulfate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 17 to 205 (189 residues), 136.8 bits, E=1.6e-43
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 19 to 424 (406 residues), 596.8 bits, E=2.2e-183
• PF22594: GTP-eEF1A_C
  • amino acids 326 to 424 (99 residues), 86.8 bits, E=3e-28
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 438 to 617 (180 residues), 244.1 bits, E=8.2e-77
• PF01583: APS_kinase
  • amino acids 454 to 602 (149 residues), 229.2 bits, E=5.5e-72
• PF13671: AAA_33
  • amino acids 458 to 569 (112 residues), 29.6 bits, E=2.1e-10

Best Hits

Swiss-Prot: 65% identical to CYSNC_XYLFA: Bifunctional enzyme CysN/CysC (cysNC) from Xylella fastidiosa (strain 9a5c)

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 68% identity to xom:XOO_3197)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (3/12 steps found)
• superpathway of seleno-compound metabolism (5/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Protein Sequence (630 amino acids)

>LRK53_RS04490 adenylyl-sulfate kinase (Rhodanobacter sp000427505 FW510-R12)
MAAPTASAITPAGAEKSLLRFITCGSVDDGKSTLLGRLLYDAGTVPDDQLAALARDSRRL
HADAGDTLDFALLTDGLDAEREQGITIDVAYRYFHTARRSFIVADCPGHEQYTRNMATGA
SNAELAVVLVDARKGLLPQTRRHTYICALLGIRQVVLAVNKMDLVDGDEAVYREISEAYR
ALAQGLGIARVECLPVIAPGGDNVGTRSARMPWYQGPSLLELLESADAAPARAADFRMPV
QWVNRPDQSFRGYAGTICGGRVAKGDEVVVQPGLQRARIERIVTADGELESAGDGQAVTL
CLDRELDASRGDVIADALRPAPVADQFTCHLLWLGDSALLPNRAYWLKLGTRTVNARVMA
IKHKVDVNSQARLAARNLELNEVGYCTLGLDEEVAFEAYADNRTLGGFILIDRQSNATVA
CGMLDFALGRSANVHWQHVDIDKSVRAASKGQQPLCLWFTGLSGAGKSTVANLVERRLHA
LGCHTYLLDGDNVRHGINRDLGFTPEDRVENIRRIAEVAHLMVDAGLIVLVSVISPYRSE
RQSARELFAASEFMEVFVDTPLEECERRDPKGLYRKARAGAIRNFTGIDAPYERPEAPDI
HLAGAGRRPEELAEQVVARIAATLDGGAED