Protein Info for LRK53_RS03980 in Rhodanobacter sp000427505 FW510-R12

Annotation: MATE family efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details amino acids 50 to 73 (24 residues), see Phobius details amino acids 93 to 113 (21 residues), see Phobius details amino acids 133 to 153 (21 residues), see Phobius details amino acids 167 to 187 (21 residues), see Phobius details amino acids 200 to 220 (21 residues), see Phobius details amino acids 241 to 270 (30 residues), see Phobius details amino acids 282 to 305 (24 residues), see Phobius details amino acids 319 to 343 (25 residues), see Phobius details amino acids 356 to 379 (24 residues), see Phobius details amino acids 392 to 412 (21 residues), see Phobius details amino acids 423 to 443 (21 residues), see Phobius details PF01554: MatE" amino acids 24 to 183 (160 residues), 97.6 bits, see alignment E=3.2e-32 amino acids 250 to 411 (162 residues), 119.8 bits, see alignment E=4.7e-39 TIGR00797: MATE efflux family protein" amino acids 24 to 424 (401 residues), 306.1 bits, see alignment E=1.8e-95

Best Hits

Swiss-Prot: 47% identical to NORM_XANCP: Probable multidrug resistance protein NorM (norM) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K03327, multidrug resistance protein, MATE family (inferred from 48% identity to smt:Smal_3598)

Predicted SEED Role

"Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (461 amino acids)

>LRK53_RS03980 MATE family efflux transporter (Rhodanobacter sp000427505 FW510-R12)
MKPFHPERAHLLHEVRATVRLALPLIAAQLAAVGSNVIDAVLAGHVSAHVLGAVAVGASI
WSLAIVCGIGMMMAVPPSVAQLDGAGRRGEVGAVFQQALWLALGMGVLLWFAVRHAQPLI
ELIGVTPSLRHDVQRFLLAISWGAPALTCYFALRGLSEGLSLTRPSMYFSLGGLVLLVPL
GYVLMFGKLGIPPQGAHGCGVATAIVLWLELLAFGVYVIFRRNYRGLGLFDRFEWPDRRR
IGALVHIGLPMAVTLLAEAGLFVATALIIATLGEDVIASHQVAINIASLFFMIPLGLAMA
ITVRVGNAVGRGDERGVRYAGFCGIGLTLLTQLFSAALMLGLPHFIASLYTHEPKVIALA
AQLIMLAGLFQFSDGIQVAANGALRGLKDTRVPMAITLFAYWMIGMPVGWWLAFHHGMGA
RGMWIGLIAGLSAAAAMLFTRFWRSAWRQRWRRPVPMTAPA