Protein Info for LRK53_RS03560 in Rhodanobacter sp000427505 FW510-R12

Annotation: glycerol-3-phosphate 1-O-acyltransferase PlsB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 60% identical to PLSB_XANCP: Glycerol-3-phosphate acyltransferase (plsB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K00631, glycerol-3-phosphate O-acyltransferase [EC: 2.3.1.15] (inferred from 59% identity to psu:Psesu_2936)

Predicted SEED Role

"Glycerol-3-phosphate acyltransferase (EC 2.3.1.15)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.3.1.15)

MetaCyc Pathways

phosphatidylglycerol biosynthesis I (6/6 steps found)
phosphatidylglycerol biosynthesis II (6/6 steps found)
superpathway of phospholipid biosynthesis III (E. coli) (10/12 steps found)
CDP-diacylglycerol biosynthesis I (4/4 steps found)
CDP-diacylglycerol biosynthesis II (4/4 steps found)
oleate biosynthesis III (cyanobacteria) (2/3 steps found)
superpathway of cardiolipin biosynthesis (bacteria) (9/13 steps found)
phosphatidate biosynthesis (yeast) (3/5 steps found)
diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
palmitoyl ethanolamide biosynthesis (2/6 steps found)
superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
stigma estolide biosynthesis (2/7 steps found)
anandamide biosynthesis II (2/8 steps found)
anandamide biosynthesis I (3/12 steps found)
superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (888 amino acids)

>LRK53_RS03560 glycerol-3-phosphate 1-O-acyltransferase PlsB (Rhodanobacter sp000427505 FW510-R12)
MTTAADSPARPRAPWWFNLAGQLLQPWVRIRRDPAEPAALLQAGVPVCYVIERDGFSDAL
ILQRACREAGLPNPMQPLAGTRRRRSVFALARRDGSLLGRNRKRNPNEALRQLVHSLEGL
PESDIQIMPVSIYVGRAPSRDSGWFRVLFSENWVMVGRFRRMLALLLNGRDTVVHFSTPV
SLRQTLEESGGITPERFARKVSRVLRTHFHRIRAAVIGPDLSHKRTVVDAVLNAEPVRAA
IAATAAKEKISHAKAWRKAHQLVLEIAADYSHPVVRSASFLLSNFWNKLYDGIAMHHFDK
ARAAAPGHEVIYVPCHRSHADYLLMSYQLHVSGVVVPHIAAGVNLDLPLIGPILRRGGAF
FLRRSFRGNALYSVVFNEYVAQLIDRGVPMEYFIEGGRSRTGRLLAPRAGMLAMTIRAFL
RAPRRPVLFQPVYIGYEKLMEGKSYIGELSGQPKEKESLLGLLKGLKVLRQRYGHVALNF
GEPIELTPLLDAASDHWRSAGTDPEAKPEWLGAVTDRLAKQIQVNINRAADVNPINLLAL
ALLATPKHAMAESDLLTQLDLGKAMLEELPYSDRITLTPMNPAAIIAYGEQMGWIQRVQH
PLGDVLTATGERAVLLSYFRNNVLHLTATAAWVACCFLNNRRMSRSSILRLGRIIYPFIQ
GELFLPWDEDGFVAQLQATIDFFVRRGLLEAVADGRVLERSPGQDDGAFQLRVIARSLIQ
AFERYYITIAALVKNGPHTLTGAELENACSLTAQRLSLLNELTAPEFFDKALFRGFIQKL
RERRIVWTDDAGKLDYDASLEGMVRDARVILSREVRHSILKITPGGDKEPAAGDPAVAPD
ALPADAAATDLHRRHVDTEQHEHERRSGSDRRLPDATEPPPTHRDAAE