Protein Info for LRK53_RS03035 in Rhodanobacter sp000427505 FW510-R12

Annotation: 8-amino-7-oxononanoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00858: 8-amino-7-oxononanoate synthase
  • amino acids 23 to 384 (362 residues), 479.4 bits, E=2.9e-148
• PF00155: Aminotran_1_2
  • amino acids 44 to 384 (341 residues), 204.4 bits, E=1.6e-64

Best Hits

Swiss-Prot: 54% identical to BIOF_CELJU: 8-amino-7-oxononanoate synthase (bioF) from Cellvibrio japonicus (strain Ueda107)

KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 57% identity to nhl:Nhal_0765)

MetaCyc: 38% identical to 2-amino-3-ketobutyrate coenzyme A ligase (Homo sapiens)
Glycine C-acetyltransferase. [EC: 2.3.1.29]

Predicted SEED Role

"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)

MetaCyc Pathways

• biotin biosynthesis I (14/15 steps found)
• 8-amino-7-oxononanoate biosynthesis I (10/11 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (5/5 steps found)
• superpathway of L-threonine metabolism (14/18 steps found)
• L-threonine degradation II (2/2 steps found)
• biotin biosynthesis II (4/6 steps found)
• 8-amino-7-oxononanoate biosynthesis II (1/2 steps found)
• 8-amino-7-oxononanoate biosynthesis III (1/2 steps found)

KEGG Metabolic Maps

• Biotin metabolism
• Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.29

Use Curated BLAST to search for 2.3.1.29 or 2.3.1.47

Protein Sequence (401 amino acids)

>LRK53_RS03035 8-amino-7-oxononanoate synthase (Rhodanobacter sp000427505 FW510-R12)
MPRPDLLQRLAGHTAEREHAQLRRRLRTIDHADGPWLESGSQRLLSFCSNDYLGLAQHPQ
LVAALTRAAREEGVGSTSAHLICGHRREHAELEEALAVWTGRERALLFSTGYMANLGVLQ
ALLARDDACVQDKLNHACLLDGAKLSGAELKRYPHADVDAAARQLAANPDAAALLATDGV
FSMDGDVAPLRELAQLCARERATLMVDDAHGLGVLGAHGAGSLDAANLGQHEVPLLMATL
GKALGCSGAFVAGPAALIDGLLQFARPYIYTTAMPPALAAAALAAVQLVQSEGWRREKLT
ALIARFRRGAAELGLPLAPSPSAIQPLLLGGADAALAAAQALERQGLLVTAIRPPTVPAG
QARLRITLSAAHEEAHIDRLLAALETLRLQAAPRASGGDHV