Protein Info for LRK53_RS02460 in Rhodanobacter sp000427505 FW510-R12

Annotation: GH1 family beta-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00232: Glyco_hydro_1
  • amino acids 7 to 446 (440 residues), 514.5 bits, E=1e-158
• TIGR03356: beta-galactosidase
  • amino acids 8 to 438 (431 residues), 581.8 bits, E=4.2e-179

Best Hits

KEGG orthology group: K05350, beta-glucosidase [EC: 3.2.1.21] (inferred from 69% identity to sal:Sala_1019)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

• neolinustatin bioactivation (2/3 steps found)
• linamarin degradation (1/2 steps found)
• lotaustralin degradation (1/2 steps found)
• linustatin bioactivation (2/4 steps found)
• cellulose degradation II (fungi) (1/3 steps found)
• coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
• α-tomatine degradation (1/6 steps found)
• firefly bioluminescence (2/14 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Phenylpropanoid biosynthesis
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Protein Sequence (451 amino acids)

>LRK53_RS02460 GH1 family beta-glucosidase (Rhodanobacter sp000427505 FW510-R12)
MPRPGFRFPDGFHWGAATSAYQIEGSPLADGAGPSIWQRFAHTPGMMANGDTGDVACDHY
RRYREDVQLMKALGLQGYRFSINWARVLPEGTGRVNPKGLDFYSRLVDALLEQGIAPNAT
LFHWDLPAALDDRGGWLNRDSAHWFAEYAEVMFKALDDRVPRWSTLNEPWVVTDGGYLHG
ALAPGHRSKYEAPIAAHNLMRASGAGIQAYRAHGKNEIGVVFNIEPKYPHSDRAEDLAAT
RRAHAYMNQQFADPALLGSYPPELKEIFGDAWPDFPADDFKLTKQKVDFVGINYYTRAVV
KHDANQYPLQAAPVRQPNKTYTETGWEVFEQGLTDTLTWFRDRYGDIPLYITENGSAFYD
PPVVEAEVLHDPLRTNYLRRHLKALHKAIEAGVNLKGYYAWSLLDNLEWSLGFSKRFGLY
HVDFTTQQRTPKATAKLYAQVIASNGSVLDD