Protein Info for LRK53_RS02305 in Rhodanobacter sp000427505 FW510-R12

Annotation: ATP-dependent protease ATPase subunit HslU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 TIGR00390: ATP-dependent protease HslVU, ATPase subunit" amino acids 4 to 444 (441 residues), 671 bits, see alignment E=4.4e-206 PF07728: AAA_5" amino acids 52 to 88 (37 residues), 24.5 bits, see alignment 7.2e-09 PF00004: AAA" amino acids 53 to 102 (50 residues), 29.6 bits, see alignment 2.5e-10 amino acids 239 to 333 (95 residues), 29.1 bits, see alignment E=3.6e-10 PF07724: AAA_2" amino acids 188 to 330 (143 residues), 86.2 bits, see alignment E=8.4e-28

Best Hits

Swiss-Prot: 72% identical to HSLU_XANCB: ATP-dependent protease ATPase subunit HslU (hslU) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K03667, ATP-dependent HslUV protease ATP-binding subunit HslU (inferred from 72% identity to xcc:XCC3493)

MetaCyc: 67% identical to ATPase component of the HslVU protease (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent hsl protease ATP-binding subunit HslU" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (444 amino acids)

>LRK53_RS02305 ATP-dependent protease ATPase subunit HslU (Rhodanobacter sp000427505 FW510-R12)
MSELTPREIVNELDRYIIGQHDAKRAVAVALRSRWRRMQLPAEMRNEVTPKNILMIGPTG
VGKTEIARRLATLANAPFVKVEATKFTEVGYVGKDVESIIRDLADVAYKLTREQAAKRVR
SQAEDRAEDRILDALLPRRQTPTDWSHDPAPTTDSDTRQKLRKQLREGALDEREIELDFA
MNVGVEIMSPPGMEEMGAQLRQMFQNIGGAKTQSRKLAIKLARPLLIDEEAGKLLNDEEV
RAQAVEAAEQNGIVFIDEIDKVAQRSDHGHAGVSREGVQRDLLPLVEGSTVSTKYGPLKT
DHMLFIASGAFSLAKPSDLIPELQGRLPIRVELSALSVDDFKRILREPHNALTKQYVALL
GTEGVTIEFTDSGIDRLAEVAFQVNERTENIGARRLHTVMERLLEKISYEAADKSGEKYL
IDAEHVDKSLGGLAKDEDLSRYIL