Protein Info for LRK53_RS02210 in Rhodanobacter sp000427505 FW510-R12

Annotation: bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 7 to 462 (456 residues), 570.9 bits, E=9.9e-176
• PF01128: IspD
  • amino acids 7 to 48 (42 residues), 28.8 bits, 2.7e-10
• PF12804: NTP_transf_3
  • amino acids 8 to 129 (122 residues), 68.1 bits, E=2.6e-22
• PF00483: NTP_transferase
  • amino acids 8 to 223 (216 residues), 37.1 bits, E=6.9e-13
• PF00132: Hexapep
  • amino acids 403 to 437 (35 residues), 36.1 bits, 8.8e-13
• PF14602: Hexapep_2
  • amino acids 405 to 437 (33 residues), 31.5 bits, see alignment (E = 2.8e-11)

Best Hits

Swiss-Prot: 63% identical to GLMU_STRM5: Bifunctional protein GlmU (glmU) from Stenotrophomonas maltophilia (strain R551-3)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 63% identity to smt:Smal_3508)

MetaCyc: 52% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• peptidoglycan recycling I (10/14 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (11/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (464 amino acids)

>LRK53_RS02210 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU (Rhodanobacter sp000427505 FW510-R12)
MNRAPLHVVILAAGAGTRMKSNRAKVLMPLAGRPLLAHVIDAARALRPAAIHVVYGHGGE
QVQAAFSGQADPGSGPGQALRWVLQAERLGTGHAVEQALAGVPDGVGVLVLYGDVPLTRA
ETLQQLAAVEGGFGLLTTRLADPHGYGRVLCDGNGRVCAVVEEKDADDGQRAVNLVNTGI
LVADAQALRGWIGRLDRNNAQGEYYLTDIFGMAAAEHRPALSVECADPVEAAGANNALQL
SELEAAYRRRATHALMAAGVRLADPLRIDVRGTVEAGRDVELDIDVILEGRVSLGDEVRV
GAFTRLKDVQLAAGTVVLPHCDLDGVVTHGPCSIGPFARLRPGTELDAGVHVGNFVETKK
THLGEGSKANHLSYLGDTVVGRGVNIGAGTITCNYDGVNKFATHIGDGAFIGSNSALVAP
VTIGAQATIGAGSVITRDAPDGELTVARGTQQTFPGWKRPVKRE