Protein Info for LRK53_RS01815 in Rhodanobacter sp000427505 FW510-R12

Annotation: acetylornithine/succinylornithine family transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 74% identical to ARGD_XANCP: Acetylornithine aminotransferase (argD) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K00821, acetylornithine/N-succinyldiaminopimelate aminotransferase [EC: 2.6.1.11 2.6.1.17] (inferred from 74% identity to xal:XALc_0776)

MetaCyc: 50% identical to succinylornithine transaminase subunit (Pseudomonas aeruginosa)
Succinylornithine transaminase. [EC: 2.6.1.81]; Ornithine aminotransferase. [EC: 2.6.1.81, 2.6.1.13]; Acetylornithine transaminase. [EC: 2.6.1.81, 2.6.1.13, 2.6.1.11]

Predicted SEED Role

"Acetylornithine aminotransferase (EC 2.6.1.11)" in subsystem Arginine Biosynthesis extended (EC 2.6.1.11)

MetaCyc Pathways

aspartate superpathway (22/25 steps found)
L-arginine biosynthesis III (via N-acetyl-L-citrulline) (9/9 steps found)
L-lysine biosynthesis I (9/9 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (15/18 steps found)
L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
L-ornithine biosynthesis I (5/5 steps found)
L-arginine degradation VI (arginase 2 pathway) (4/4 steps found)
L-arginine degradation I (arginase pathway) (3/3 steps found)
L-ornithine biosynthesis II (3/3 steps found)
L-proline biosynthesis III (from L-ornithine) (3/3 steps found)
L-arginine biosynthesis II (acetyl cycle) (8/10 steps found)
L-citrulline biosynthesis (6/8 steps found)
L-N^δ-acetylornithine biosynthesis (5/7 steps found)
superpathway of arginine and polyamine biosynthesis (12/17 steps found)
L-arginine degradation XIII (reductive Stickland reaction) (3/5 steps found)
superpathway of L-citrulline metabolism (8/12 steps found)
L-arginine degradation II (AST pathway) (1/5 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.11

Use Curated BLAST to search for 2.6.1.11 or 2.6.1.13 or 2.6.1.17 or 2.6.1.81

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (411 amino acids)

>LRK53_RS01815 acetylornithine/succinylornithine family transaminase (Rhodanobacter sp000427505 FW510-R12)
MTRPDATTEALISLGKRYWLPVYKPRELVLDHGKGARVWDTEGRDYVDFGAGIAVNALGH
QDPDLLAALTAQAHKLWHSSNVFYTEPPLRLAEELVQASGFAERVFLCNSGAEANEAAIK
LVRKWAAAQGRAPERRVIVTFNGSFHGRTLATVTATAQPKYQQGYEPLPAGFRYLDFNDA
AALEAAFAAGDIAAVMLEPVQGEGGVVPAAPGFLKRVRELCDAHGALLVLDEIQCGMGRT
GTLFAHAHDNVAPDIVTLAKALGGGFPIGAMLAGPKVAEVMQYGAHGTTFGGNPMAAAVA
RVALAKLSSSAVLMNVERQANDLRAGLARINHELQLFDEVRGRGLMIGAALAEAHRGKAG
AILDHAAAHGLLLLQAGPDVLRFVPPLTITDEELADGLGRLHAALSDFAAG