Protein Info for LRK53_RS01075 in Rhodanobacter sp000427505 FW510-R12

Annotation: DEAD/DEAH box helicase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 818 PF13588: HSDR_N_2" amino acids 33 to 112 (80 residues), 28.4 bits, see alignment E=4.4e-10 PF04313: HSDR_N" amino acids 52 to 113 (62 residues), 30.9 bits, see alignment 7.3e-11 PF04851: ResIII" amino acids 164 to 344 (181 residues), 153.3 bits, see alignment E=1.7e-48 PF18766: SWI2_SNF2" amino acids 169 to 378 (210 residues), 61.1 bits, see alignment E=3.8e-20 PF00270: DEAD" amino acids 170 to 345 (176 residues), 42.2 bits, see alignment E=2.2e-14 PF08463: EcoEI_R_C" amino acids 659 to 814 (156 residues), 111.8 bits, see alignment E=1.1e-35

Best Hits

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 72% identity to nwi:Nwi_1255)

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (818 amino acids)

>LRK53_RS01075 DEAD/DEAH box helicase family protein (Rhodanobacter sp000427505 FW510-R12)
MDAGAKLSERDVCTKFITPALVAAGWDVQTQIREEVGFTKGRIIVRGRLVTRGKAKRADY
VLYYKHLPLALIEAKDQTHAAGDGMQQALGYATTLDIPYVFSSNGKGFQFHDRSGVSVPL
EQSLSMDEFPSPNELWSRYRSWKGLQPAQESTVLQAYYEDGSGKEPRYYQRNAINAAVEA
IAKGQNRILLVMATGTGKTYTAFQIIWRLWKAGQKKRILFLADRNVLVDQTMVNDFRPFG
PAMAKLSTASKTIERADGTEIELTLALDKRRRIDPAYEIYLGLYQAITGPEERQKLFREF
SPDFFDLIVIDECHRGSAAEDSAWREILEYFSSATQIGLTATPKETEYVSNIHYFGEPVY
TYSLKQGISDGFLAPYKVIKVHLDVDVQGYRPKSGETDKHGYLIDDRLYNEKDFDRTLVI
DERTQRVAKWISDYLKQSGDRFQKTIVFCVDTEHAARMRQALINENADLVQQNARYVMRI
TGDDNEGTAQLGNFIDPESPYPVIVTTSRLLSTGVDAQTCRLIVLDREVGSMTEFKQIVG
RGTRVHEDTHKYYFTLVDFRKATNHFADPDFDGEPVQIYQPDEYDSPVPPEDVPLDEEGN
PLPSQPTGEETIVDQPPPAIPPPGVDDDQPRKFYVKQRPVTVVLERVEYLDESGKLVTES
LRDYSRNAIRRHFTSLDAFLRRWREAERKESVIAELAEEGLLLEPLQEEVGKDLDPFDLI
CHIAFDQPPLSRRERADSVTKRDVFTKYGPQACAVLEALLAKYRDEGVVTDLDNVRVLEI
PPFNAMGTPIQLIKPFGSKAGFENAVHELQNALYDETA